Delgado, Paula E.

Kim, Jennifer J.

English

ScholarWorks @ Georgia State University

TITLE: Investigating the active ingredient in Eupatorium capillifolium anti-quorum sensing properties  AUTHORS: Jennifer Kim & Paula Delgado   FACULTY SPONSOR: Dr. Maria Nagy, Instructor, Department of Biology   Introduction: Eupatorium capillifolium is a plant that grows abundantly in the southeast of the United States, and it is commonly known as dog fennel. Dog fennel is recognized as a weed, being very resistant in nature, as it requires minimal conditions to thrive. E. capillifolium has shown some medicinal uses according to past folklore, and is also used as an insect repellant in present day. Previous research on E. capillifolium has suggested that it has anti-quorum sensing capabilities against Chromobacterium violaceum. C. violaceum is a gram negative bacteria that is not normally detrimental to human health; however, for those immuno-compromised, it may have harmful effects. Like that of many other bacterial species, C. violaceum, uses quorum sensing to maintain its virulence.   Purpose: This study will attempt to find the active ingredient of E. capillifolium with anti-quorum sensing properties.   Method: This experiment will be carried out utilizing TLC bioassays, column chromatography and activity-driven fractionations.   Results: The initial results of the TLC bioassay showed E. capillifolium leaf extract formed zones of inhibition at a RF range of 0.083-0.33. Column chromatography was utilized to further separate the plant extract compounds, and furthermore, activity-driven fractionations will be used to determine specific compounds of E. capillifolium that inhibits quorum sensing in C. violaceum.   Conclusion: The rise of multi-resistant bacteria is a common concern among those in the healthcare industry. Alternative methods of combating pathogenic bacteria are deeply investigated as they may solve problems of medicine. An answer to these ordeals may be answered with the use of plants as natural alternatives and their innate anti-quorum sensing properties.    

Investigating the active ingredient in Eupatorium capillifolium anti-quorum sensing properties

Georgia State University

TITLE: Investigating the active ingredient in Eupatorium capillifolium anti-quorum 
sensing properties 
 
AUTHORS: Jennifer Kim & Paula Delgado  
 
FACULTY SPONSOR: Dr. Maria Nagy, Instructor, Department of Biology  
 
Introduction: Eupatorium capillifolium is a plant that grows abundantly in the 
southeast of the United States, and it is commonly known as dog fennel. Dog fennel 
is recognized as a weed, being very resistant in nature, as it requires minimal 
conditions to thrive. E. capillifolium has shown some medicinal uses according to 
past folklore, and is also used as an insect repellant in present day. Previous 
research on E. capillifolium has suggested that it has anti-quorum sensing 
capabilities against Chromobacterium violaceum. C. violaceum is a gram negative 
bacteria that is not normally detrimental to human health; however, for those 
immuno-compromised, it may have harmful effects. Like that of many other 
bacterial species, C. violaceum, uses quorum sensing to maintain its virulence.  
 
Purpose: This study will attempt to find the active ingredient of E. capillifolium with 
anti-quorum sensing properties.  
 
Method: This experiment will be carried out utilizing TLC bioassays, column 
chromatography and activity-driven fractionations.  
 
Results: The initial results of the TLC bioassay showed E. capillifolium leaf extract 
formed zones of inhibition at a RF range of 0.083-0.33. Column chromatography was 
utilized to further separate the plant extract compounds, and furthermore, activity-
driven fractionations will be used to determine specific compounds of E. 
capillifolium that inhibits quorum sensing in C. violaceum.  
 
Conclusion: The rise of multi-resistant bacteria is a common concern among those 
in the healthcare industry. Alternative methods of combating pathogenic bacteria 
are deeply investigated as they may solve problems of medicine. An answer to these 
ordeals may be answered with the use of plants as natural alternatives and their 
innate anti-quorum sensing properties.  
 
 


https://scholarworks.gsu.edu/cgi/viewcontent.cgi?article=1312&amp;context=gsurc

Investigating the active ingredient in Eupatorium capillifolium anti-quorum sensing properties

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