Póster presentado en: 17th European Meeting on Supercritical Fluids. 2019, 8-11 de abril, Ciudad Real(MINECO) and the European Regional Development Fund (ERDF) for financial support of project CTQ2015-64396-

Illera Gigante, Alba Ester

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Repositorio Institucional de la Universidad de Burgos

April 2019 17th European Meeting on Supercritical Fluids - 7th European Meeting on High Pressure Technology ITQUIMA: Institute of Chemical and Environmental Technology Av. Camilo José Cela, 1, 13005 Ciudad Real (Spain)  A.E. Illeraa, M.T. Sanza, L. Castroa, O. Benito-Romána, S. Beltrána, R. Melgosaa & C. Apetreia aUniversity of Burgos, Department of Biotechnology and Food Science. Chemical Engineering Division. tersanz@ubu.es bChemistry, Physics and Environment Department University of Galati, Romania Acknowledgements • To MINECO and ERDF for financial support of AEI’s contract through project CTA2015-64936-R • To JCyL and ERDF for financial support of project BU301P18 and O. Benito-Román’s post-doctoral contract. Introduction: Melanosis in Litopenaeus vannamei EUROPEAN REGIONAL DEVELOPMENT FUND EUROPEAN SOCIAL FUND Inactivation of polyphenoloxidase from Litopenaeus vannamei  by High Pressure Carbon Dioxide (HPCD) To avoid melanosis in shrimps, sulphites are usually employed; however, their use is associated to allergies and asthmatic attacks. In this work, the use of HPCD is proposed as a cold pasteurization method, with temperatures below 50 ºC and pressures lower than 50 MPa. Mechanism of polyphenol oxidase (PPO) OHOHOHNHHNOOOHHOR ROORPPO + O2Monophenol O-DiphenolMonophenolase activityPPO + O2Diphenolase activityO-QuinoneNon-enzymatic oxidative condensationBrown melanoidin pigmentsPhenols, proteins, amino acidsReducing agentExperimental section 1: CO2 supply 2: Syringe pump and controller (ISCO 260D) 3: Cooling system 4: Rupture disc 5: High pressure vessel 6: Thermostatic bath 7: Magnetic stirrer 8: Sampling system Preparation of a crude PPO extract: heads of Litopenaeus vannamei  crushed with a blender and liquid N2 in phosphate buffer (pH = 7.2) with 0.2 % Brij 35  filtered  kept in freezing conditions until treatment and analysis.  Determination of enzymatic inactivation kinetics of PPO extracts: Data fitted to the Weibull model  Treatment of whole shrimp pieces by HPCD: Determination of PPO activity of visual colour observation. Results and discussion Literature: [1] A. E. Illera et al. J. Food Eng. 2018, 239, 64–71 [2] L. Zhou et al. Innov. Food Sci. Emerg. Technol. 2009, 10, 321–327  Inactivation kinetic data of PPO extract 0204060801001200 10 20 30 40 50 60PPO residual activity (%) Time (minutes) Inactivation kinetics of PPO extract by HPCD at different T  Lines represent the calculated values for the Weibull model. T, ºC α, min β R2 td=1, min 35 40 50 69 ± 9 30 ± 3 16 ± 2 0.48 ± 0.06 0.53 ± 0.07 0.46 ± 0.07 0.984 0.982 0.984 399 145 95 p = 20 MPa Faster inactivation at higher T: intrinsic effect of T and better transport properties of CO2. PPO inactivation in whole shrimp pieces Treatment Mild heating HPCD PPO residual activity  83.3 % Non detected PPO residual activity after HPCD and mild heating treatment (MH) at 40 ºC. PPO was extracted at pH =7.2 with 0.2 % Brij 35 at 4 ºC.  Shrimps stored in plastic bag at 4 ºC during 7 days:   Untreated shrimps showed up browning in the cephalothorax after the second day.  HPCD treated shrimps presented an aspect similar to cooked shrimps. Light browning in cephalothorax appeared in the fifth days. logAAo=−12.303𝑡𝑡𝛼𝛼𝛽𝛽 𝑡𝑡𝑑𝑑 = 𝛼𝛼 −𝑙𝑙𝑙𝑙 10−𝑑𝑑1𝛽𝛽  

Inactivation of polyphenoloxidase from Litopenaeus vannamei by High Pressure Carbon Dioxide

https://riubu.ubu.es/bitstream/10259/5168/1/Inactivation_of_polyphenoloxidase_from.pdf

Inactivation of polyphenoloxidase from Litopenaeus vannamei by High Pressure Carbon Dioxide

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