Axton, Richard

Burdon, Tom

Fidanza, Antonella

Forrester, Lesley M

Jackson, Melany

Kydonaki, Maria

Medvinsky, Alexander

Meek, Stephen

Rybtsov, Stanislav

Taylor, Helen

English

Apelin receptor (Aplnr/Agtrl1/Apj) marks a transient cell population during the differentiation of hematopoietic stem and progenitor cells (HSPCs) from pluripotent stem cells but its function during the production and maintenance of haematopoietic stem cells is not clear. We generated an Aplnr-tdTomato reporter mouse embryonic stem cell (mESC) line and showed that HSPCs are generated exclusively from mesodermal cells that express Aplnr. HSPCs production from mESCs was impaired when Aplnr was deleted implying that this pathway is required for their production. To address the role of the Aplnr pathway in HSPC maintenance, we added Apelin ligands to ex vivo AGM cultures. Activation of the Aplnr pathway in this system impaired the generation of long-term reconstituting HSPCs and appeared to drive myeloid differentiation. Our data suggest that the Aplnr signalling is required for the generation of cells that give rise to HSCs but its subsequent down regulation is required for their maintenanc

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Modulation of Aplnr signaling is required during the development and maintenance of the hematopoietic system

https://www.research.ed.ac.uk/files/199046510/PIIS2213671121000837.pdf

Modulation of Aplnr signaling is required during the development and maintenance of the hematopoietic system

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