Ballantyne, Maeve

Doddamani, Dadakhalandar

Hawken, Rachel

Hu, Tuanjun

McGrew, Mike

Taylor, Lorna

Woodcock, Mark

English

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Single generation allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

Poultry is the most abundant livestock species with over 60 billion chickens raised globally per year. While most chicken are produced from highly selected commercial flocks the many indigenous chicken breeds, which have low productivity and have not been highly selected, play an important role in rural economies across the world as they are well adapted to local environmental and scavenging conditions. The ability to rapidly transfer genetic changes between breeds of chicken will permit the transfer of beneficial alleles between poultry breeds as well as allow validation of genetic variants responsible for different phenotypic traits. Here, we generate a novel inducibly sterile surrogate host chicken. Introducing donor genome edited primordial cells into the sterile male and female host embryos produces chicken carrying only exogenous germ cells. Subsequent direct mating of the surrogate hosts, Sire Dam Surrogate (SDS) mating, recreates pure chicken breeds carrying the edited allele in heterozygous or homozygous states. We demonstrate the transfer and validation of two feather trait alleles, Dominant white and Frizzle traits into two pure chicken breeds using the SDS surrogate hosts. This technology will allow the rapid reconstitution of chicken breeds carrying desired genetic changes to investigate climate adaptation and disease resilience traits

Edinburgh Research Explorer

https://www.research.ed.ac.uk/portal/files/164723187/Single_generation_allele_introgression_into_pure_chicken_breeds_using_Sire_Dam_Surrogate.pdf

Single generation allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating

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