Alexander J. Martinko

Amy Hu

Di Palma

Huimin Geng

James A. Wells

Jarrett J. Adams

Jason Moffat

Lynda Ploder

Markus Müschen

Mezquita

Michael Hornsby

Mummery

Olivier Julien

Sachdev S. Sidhu

Samuel B. Pollock

Yun Mou

Significance
          Next-generation sequencing (NGS) has allowed the comprehensive study of the genome and transcriptome. However, a similarly broad, highly multiplexed, and inexpensive method for proteomics using NGS remains elusive. Here, we describe a phage display-based method using preselected antibodies that are genetically encoded and capable of simultaneous profiling of hundreds of cell-surface targets on cells in culture or singly at low cost and without the need for chemical conjugation to purified antibodies. We use the method to identify cell-surface proteins that change in cancer cells, some of which are coordinately regulated and could lead to new biomarkers and cancer targets.</jats:p

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Proceedings of the National Academy of Sciences

Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies

Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies

Abstract

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