Chaim

Guerniou

Isaac A. Chaim

Jennifer J. Jordan

Le P. Ngo

Leona D. Samson

Patrizia Mazzucato

Zachary D. Nagel

Significance
          The DNA in each cell is damaged thousands of times daily. Consequently, a battery of DNA repair pathways exist that allow repair of this damage. Failure to repair can lead to devastating diseases, including cancer and neurodegeneration. Each individual’s DNA repair capacity (DRC) is inherently different. Being able to measure an individual’s DRC could contribute to a personalized approach to prevent and treat disease. Here we present powerful tools for measuring in vivo base excision repair capacity for five distinct DNA lesions. We use these methods to predict the cellular responses to a variety of DNA damaging agents, and to monitor differences in DRC in primary human lymphocytes. Additionally, we unveil previously unknown transcriptional mutagenesis induced by DNA lesions.</jats:p

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Proceedings of the National Academy of Sciences

In vivo measurements of interindividual differences in DNA glycosylases and APE1 activities

In vivo measurements of interindividual differences in DNA glycosylases and APE1 activities

Abstract

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