Calvert, Jay G

Cao, Dianjun

Cao, Qian

Halbur, Patrick G

Lynn Heffron, C

Meng, Xiang-Jin

Ni, Yan-Yan

Opriessnig, Tanja

Overend, Christopher

Pearce, Douglas S

Piñeyro, Pablo

Tian, Debin

Yugo, Danielle M

Zhou, Lei

The extensive genetic diversity of porcine reproductive and respiratory syndrome virus (PRRSV) strains is a major obstacle for vaccine development. We previously demonstrated that chimeric PRRSVs in which a single envelope gene (ORF3, ORF4, ORF5 or ORF6) was shuffled via DNA shuffling had an improved heterologous cross-neutralizing ability. In this study, we incorporate all of the individually-shuffled envelope genes together in different combinations into an infectious clone backbone of PRRSV MLV Fostera(®) PRRS. Five viable progeny chimeric viruses were rescued, and their growth characteristics were characterized in vitro. In a pilot pig study, two chimeric viruses (FV-SPDS-VR2,FV-SPDS-VR5) were found to induce cross-neutralizing antibodies against heterologous strains. A subsequent vaccination/challenge study in 72 pigs revealed that chimeric virus FV-SPDS-VR2 and parental virus conferred partial cross-protection when challenged with heterologous strains NADC20 or MN184B. The results have important implications for future development of an effective PRRSV vaccine that confers heterologous protection.</p

Edinburgh Research Explorer

Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

Debin Tian

Yan-Yan Ni

Lei Zhou

Tanja Opriessnig

Dianjun Cao

Pablo Piñeyro

Danielle M. Yugo

Christopher Overend

Qian Cao

C. Lynn Heffron

Patrick G. Halbur

Douglas S. Pearce

Jay G. Calvert

Xiang-Jin Meng

Crossref

Virology

https://www.pure.ed.ac.uk/ws/files/22932659/Tian_Virology.pdf

Chimeric porcine reproductive and respiratory syndrome virus containing shuffled multiple envelope genes confers cross-protection in pigs

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