Alam, Shirjel

Dweck, Marc R

Gray, Calum D

Henriksen, Peter a

Macgillivray, Thomas J

Mckillop, Graham

Mirsadraee, Saeed

Newby, David

Semple, Scott

Stirrat, Colin G

Journal of Cardiovascular Magnetic Resonance

PubMed

Springer - Publisher Connector

POSTER PRESENTATION Open Access
Ultrasmall superparamagnetic particles of iron
oxide-enhanced magnetic resonance imaging in
the assessment of cellular inflammation after
myocardial infarction
Colin G Stirrat2,1*, Shirjel Alam2,1, Thomas J MacGillivray1,3, Calum D Gray1,3, Marc R Dweck2,1, Saeed Mirsadraee1,4,
Graham McKillop4, Peter A Henriksen2,1, David Newby2,1, Scott Semple1,2
From 18th Annual SCMR Scientific Sessions
Nice, France. 4-7 February 2015
Background
Excessive inflammation after myocardial infarction (MI)
can be detrimental to the recovery of cardiac function.1,2
Ultrasmall superparamagnetic particles of iron oxide
(USPIO)-enhanced magnetic resonance imaging (MRI)
can be used to detect myocardial cellular inflammation.
USPIO are engulfed by resident macrophages through
phagocytosis and pinocytosis resulting in concentration
within inflamed tissues. We aimed to determine the time
course and duration of USPIO-enhancement following
acute MI (Figure 1).
Methods
Thirty patients with acute MI were studied in the 3-month
period following acute MI. Repeated T2*-weighted 3T
MRI was performed immediately before and 24 h after
USPIO (ferumoxytol, 4 mg/kg) administration at 2±1, 5±2,
13±3, 21±4 and 90±9 days. Myocardial regions of interest
(ROIs) were drawn and categorised into infarct and non-
infarct regions by the presence or absence of late gadoli-
nium enhancement (LGE). R2* values (1/T2*) within ROIs
were determined to assess the time course and duration of
uptake of USPIO.
Results
Following single-dose USPIO administration 2-7 days after
acute MI, USPIO uptake is demonstrable at 24 h (p<0.001)
and is cleared within 4-8 days. Increased USPIO uptake is
seen in the infarct region at days 2-3 (p<0.001), days 4-7
(p<0.01), and days 10-16 (p<0.05) compared to non-
2Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK
Full list of author information is available at the end of the article
Figure 1 Patient imaged 5 days after an inferior MI. Images show late gadolinium enhancement in the region of the MI (left). R2* colour maps
of the baseline pre-USPIO scan (middle), and 24 hours following USPIO administration (right). USPIO uptake, with increased R2* values, is shown
by the lighter area of the colour map in the region of the inferior wall infarct (arrow).
Stirrat et al. Journal of Cardiovascular Magnetic
Resonance 2015, 17(Suppl 1):P252
http://www.jcmr-online.com/content/17/S1/P252
© 2015 Stirrat et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in
any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://
creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
infarcted myocardium (Figure 2). There was no difference
in uptake between regions at later time points (21±4 and
90±9 days).
Conclusions
USPIO-enhanced MRI can detect and quantify infarct-
related cellular inflammation in the first 2 weeks following
acute MI. This imaging tool holds promise to non-inva-
sively assess myocardial cellular inflammation after MI
and in other inflammatory cardiac conditions. It has the
potential to monitor disease progression and remission,
and may provide a future platform on which to test novel
anti-inflammatory therapies for the heart.
Funding
The BHF have provided funding for this work (FS/12/
83/29781).
Authors’ details
1Clinical Research Imaging Centre, University of Edinburgh, Edinburgh, UK.
2Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.
3Wellcome Trust Clinical Research Facility, NHS Lothian, Edinburgh, UK.
4Department of Radiology, NHS Lothian, Edinburgh, UK.
Published: 3 February 2015
doi:10.1186/1532-429X-17-S1-P252
Cite this article as: Stirrat et al.: Ultrasmall superparamagnetic particles
of iron oxide-enhanced magnetic resonance imaging in the assessment
of cellular inflammation after myocardial infarction. Journal of
Cardiovascular Magnetic Resonance 2015 17(Suppl 1):P252.
Submit your next manuscript to BioMed Central
and take full advantage of: 
• Convenient online submission
• Thorough peer review
• No space constraints or color figure charges
• Immediate publication on acceptance
• Inclusion in PubMed, CAS, Scopus and Google Scholar
• Research which is freely available for redistribution
Submit your manuscript at 
www.biomedcentral.com/submit
Figure 2 Increased USPIO uptake is seen in the infarct region at days 2-3 (p<0.001), 4-7 (p<0.01), and days 10-16 (p<0.05) compared to non-
infarcted myocardium. There is no difference in uptake at later time points (Days 81-99 not shown).
Stirrat et al. Journal of Cardiovascular Magnetic
Resonance 2015, 17(Suppl 1):P252
http://www.jcmr-online.com/content/17/S1/P252
Page 2 of 2


Ultrasmall superparamagnetic particles of iron oxide-enhanced magnetic resonance imaging in the assessment of cellular inflammation after myocardial infarction

Colin G Stirrat

Shirjel Alam

Thomas J MacGillivray

Calum D Gray

Marc R Dweck

Saeed Mirsadraee

Graham McKillop

Peter A Henriksen

David Newby

Scott Semple

Crossref

Edinburgh Research Explorer

     Edinburgh Research Explorer                                      Ultrasmall superparamagnetic particles of iron oxide-enhancedmagnetic resonance imaging in the assessment of cellularinflammation after myocardial infarctionCitation for published version:Stirrat, CG, Alam, S, Macgillivray, TJ, Gray, CD, Dweck, MR, Mirsadraee, S, Mckillop, G, Henriksen, PA,Newby, D & Semple, S 2015, 'Ultrasmall superparamagnetic particles of iron oxide-enhanced magneticresonance imaging in the assessment of cellular inflammation after myocardial infarction' pp. P252. DOI:10.1186/1532-429X-17-S1-P252Digital Object Identifier (DOI):10.1186/1532-429X-17-S1-P252Link:Link to publication record in Edinburgh Research ExplorerDocument Version:Publisher's PDF, also known as Version of recordPublisher Rights Statement: 2015 Stirrat et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of theCreative CommonsAttribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, andreproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedicationwaiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwisestatedGeneral rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s)and / or other copyright owners and it is a condition of accessing these publications that users recognise andabide by the legal requirements associated with these rights.Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorercontent complies with UK legislation. If you believe that the public display of this file breaches copyright pleasecontact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately andinvestigate your claim.Download date: 05. Apr. 2019POSTER PRESENTATION Open AccessUltrasmall superparamagnetic particles of ironoxide-enhanced magnetic resonance imaging inthe assessment of cellular inflammation aftermyocardial infarctionColin G Stirrat2,1*, Shirjel Alam2,1, Thomas J MacGillivray1,3, Calum D Gray1,3, Marc R Dweck2,1, Saeed Mirsadraee1,4,Graham McKillop4, Peter A Henriksen2,1, David Newby2,1, Scott Semple1,2From 18th Annual SCMR Scientific SessionsNice, France. 4-7 February 2015BackgroundExcessive inflammation after myocardial infarction (MI)can be detrimental to the recovery of cardiac function.1,2Ultrasmall superparamagnetic particles of iron oxide(USPIO)-enhanced magnetic resonance imaging (MRI)can be used to detect myocardial cellular inflammation.USPIO are engulfed by resident macrophages throughphagocytosis and pinocytosis resulting in concentrationwithin inflamed tissues. We aimed to determine the timecourse and duration of USPIO-enhancement followingacute MI (Figure 1).MethodsThirty patients with acute MI were studied in the 3-monthperiod following acute MI. Repeated T2*-weighted 3TMRI was performed immediately before and 24 h afterUSPIO (ferumoxytol, 4 mg/kg) administration at 2±1, 5±2,13±3, 21±4 and 90±9 days. Myocardial regions of interest(ROIs) were drawn and categorised into infarct and non-infarct regions by the presence or absence of late gadoli-nium enhancement (LGE). R2* values (1/T2*) within ROIswere determined to assess the time course and duration ofuptake of USPIO.ResultsFollowing single-dose USPIO administration 2-7 days afteracute MI, USPIO uptake is demonstrable at 24 h (p<0.001)and is cleared within 4-8 days. Increased USPIO uptake isseen in the infarct region at days 2-3 (p<0.001), days 4-7(p<0.01), and days 10-16 (p<0.05) compared to non-2Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UKFull list of author information is available at the end of the articleFigure 1 Patient imaged 5 days after an inferior MI. Images show late gadolinium enhancement in the region of the MI (left). R2* colour mapsof the baseline pre-USPIO scan (middle), and 24 hours following USPIO administration (right). USPIO uptake, with increased R2* values, is shownby the lighter area of the colour map in the region of the inferior wall infarct (arrow).Stirrat et al. Journal of Cardiovascular MagneticResonance 2015, 17(Suppl 1):P252http://www.jcmr-online.com/content/17/S1/P252© 2015 Stirrat et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.infarcted myocardium (Figure 2). There was no differencein uptake between regions at later time points (21±4 and90±9 days).ConclusionsUSPIO-enhanced MRI can detect and quantify infarct-related cellular inflammation in the first 2 weeks followingacute MI. This imaging tool holds promise to non-inva-sively assess myocardial cellular inflammation after MIand in other inflammatory cardiac conditions. It has thepotential to monitor disease progression and remission,and may provide a future platform on which to test novelanti-inflammatory therapies for the heart.FundingThe BHF have provided funding for this work (FS/12/83/29781).Authors’ details1Clinical Research Imaging Centre, University of Edinburgh, Edinburgh, UK.2Centre for Cardiovascular Science, University of Edinburgh, Edinburgh, UK.3Wellcome Trust Clinical Research Facility, NHS Lothian, Edinburgh, UK.4Department of Radiology, NHS Lothian, Edinburgh, UK.Published: 3 February 2015doi:10.1186/1532-429X-17-S1-P252Cite this article as: Stirrat et al.: Ultrasmall superparamagnetic particlesof iron oxide-enhanced magnetic resonance imaging in the assessmentof cellular inflammation after myocardial infarction. Journal ofCardiovascular Magnetic Resonance 2015 17(Suppl 1):P252.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submitFigure 2 Increased USPIO uptake is seen in the infarct region at days 2-3 (p<0.001), 4-7 (p<0.01), and days 10-16 (p<0.05) compared to non-infarcted myocardium. There is no difference in uptake at later time points (Days 81-99 not shown).Stirrat et al. Journal of Cardiovascular MagneticResonance 2015, 17(Suppl 1):P252http://www.jcmr-online.com/content/17/S1/P252Page 2 of 2

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Ultrasmall superparamagnetic particles of iron oxide-enhanced magnetic resonance imaging in the assessment of cellular inflammation after myocardial infarction

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