A chemically defined hydrogel HG21, which allows enzyme-free passaging, is a substitute for gelatin allowing standardised and inexpensive mESC culture.</p

Bradley, Mark

de Sousa, Paul A

Lilienkampf, Annamaria

Mangani, Christian

Roy, Marcia

A chemically defined thermoresponsive hydrogel, poly(AEtMA-Cl-co-DEAEA) cross-linked with N,N'-methylenebisacrylamide, which allows enzyme-free passaging, was used as a substrate to culture murine embryonic stem cells (mESCs) under defined and undefined conditions. Analysis of 14 stem cell markers showed that the mESCs remained in a "naïve" state of pluripotency with differentiation potential to form endoderm, mesoderm, and ectoderm derived lineages. These results validate the use of a chemically defined hydrogel for standardised and inexpensive mESC culture.</p

Edinburgh Research Explorer

Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

Christian Mangani

Annamaria Lilienkampf

Marcia Roy

Paul A. de Sousa

Mark Bradley

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Biomaterials Science

Thermoresponsive hydrogel maintains the mouse embryonic stem cell “naïve” pluripotency phenotype

https://www.pure.ed.ac.uk/ws/files/23388100/C5BM00121H.pdf

Thermoresponsive hydrogel maintains the mouse embryonic stem cell "naïve" pluripotency phenotype

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