Macintyre, Im

Phillips, La

Webb, Dj

PubMed

LA Phillips

IM MacIntyre

DJ Webb

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BMC Proceedings

Development of an in vivo protocol for investigating natriuretic mechanisms in response to acute sodium loading in humans

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ORAL PRESENTATION Open Access
Development of an in vivo protocol for
investigating natriuretic mechanisms in response
to acute sodium loading in humans
LA Phillips*, IM MacIntyre, DJ Webb
From International Conference for Healthcare and Medical Students 2011
Dublin, Ireland. 4-5 November 2011
Introduction
Extracellular fluid volume and arterial blood pressure are
principally determined by total body sodium via osmore-
gulation. Dysregulation of mechanisms involved in main-
taining sodium balance are therefore implicated in the
development of hypertension. Hormonal mechanisms are
thought to be critically involved in the regulation of
natriuresis, and modification of these mechanisms may be
a useful strategy in managing hypertension. Our aim was
to establish an appropriate protocol for inducing natriur-
esis, which could be used to investigate the role of various
hormone systems in maintaining sodium balance, and the
effects of using pharmacological agents to manipulate
these systems.
Methods
0.9% Saline was administered to 6 healthy subjects at a
rate of 500ml/hour over a period of two hours.
Results
Significant natriuresis occurred within 2 hours of sodium
loading (p<0.01) and was induced independently of per-
ipheral blood pressure.
Conclusions
By demonstrating that significant natriuresis can occur
within the timeframe of our protocol, and that it is inde-
pendent of non-hormonal mechanisms such as pressure
natriuresis, we have developed a protocol that can be
applied to the investigation and subsequent manipulation
of hormonal control of natriuresis. We aim to use this
protocol to study the role of the endothelin system in
natriuresis.
Published: 9 July 2012
doi:10.1186/1753-6561-6-S4-O45
Cite this article as: Phillips et al.: Development of an in vivo protocol for
investigating natriuretic mechanisms in response to acute sodium
loading in humans. BMC Proceedings 2012 6(Suppl 4):O45.
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© 2012 Phillips et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons
Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in
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Phillips LA

MacIntyre IM

Webb DJ

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     Edinburgh Research Explorer                                      Development of an in vivo protocol for investigating natriureticmechanisms in response to acute sodium loading in humansCitation for published version:Phillips, L, Macintyre, I & Webb, D 2012, 'Development of an in vivo protocol for investigating natriureticmechanisms in response to acute sodium loading in humans' BMC Proceedings, vol. 6, no. Suppl 4, pp.O45. DOI: 10.1186/1753-6561-6-S4-O45Digital Object Identifier (DOI):10.1186/1753-6561-6-S4-O45Link:Link to publication record in Edinburgh Research ExplorerDocument Version:Publisher's PDF, also known as Version of recordPublished In:BMC ProceedingsPublisher Rights Statement:© 2012 Phillips et al; licensee BioMed Central Ltd.This is an Open Access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.General rightsCopyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s)and / or other copyright owners and it is a condition of accessing these publications that users recognise andabide by the legal requirements associated with these rights.Take down policyThe University of Edinburgh has made every reasonable effort to ensure that Edinburgh Research Explorercontent complies with UK legislation. If you believe that the public display of this file breaches copyright pleasecontact openaccess@ed.ac.uk providing details, and we will remove access to the work immediately andinvestigate your claim.Download date: 05. Apr. 2019ORAL PRESENTATION Open AccessDevelopment of an in vivo protocol forinvestigating natriuretic mechanisms in responseto acute sodium loading in humansLA Phillips*, IM MacIntyre, DJ WebbFrom International Conference for Healthcare and Medical Students 2011Dublin, Ireland. 4-5 November 2011IntroductionExtracellular fluid volume and arterial blood pressure areprincipally determined by total body sodium via osmore-gulation. Dysregulation of mechanisms involved in main-taining sodium balance are therefore implicated in thedevelopment of hypertension. Hormonal mechanisms arethought to be critically involved in the regulation ofnatriuresis, and modification of these mechanisms may bea useful strategy in managing hypertension. Our aim wasto establish an appropriate protocol for inducing natriur-esis, which could be used to investigate the role of varioushormone systems in maintaining sodium balance, and theeffects of using pharmacological agents to manipulatethese systems.Methods0.9% Saline was administered to 6 healthy subjects at arate of 500ml/hour over a period of two hours.ResultsSignificant natriuresis occurred within 2 hours of sodiumloading (p<0.01) and was induced independently of per-ipheral blood pressure.ConclusionsBy demonstrating that significant natriuresis can occurwithin the timeframe of our protocol, and that it is inde-pendent of non-hormonal mechanisms such as pressurenatriuresis, we have developed a protocol that can beapplied to the investigation and subsequent manipulationof hormonal control of natriuresis. We aim to use thisprotocol to study the role of the endothelin system innatriuresis.Published: 9 July 2012doi:10.1186/1753-6561-6-S4-O45Cite this article as: Phillips et al.: Development of an in vivo protocol forinvestigating natriuretic mechanisms in response to acute sodiumloading in humans. BMC Proceedings 2012 6(Suppl 4):O45.Submit your next manuscript to BioMed Centraland take full advantage of: • Convenient online submission• Thorough peer review• No space constraints or color figure charges• Immediate publication on acceptance• Inclusion in PubMed, CAS, Scopus and Google Scholar• Research which is freely available for redistributionSubmit your manuscript at www.biomedcentral.com/submitUniversity of Edinburgh, UKPhillips et al. BMC Proceedings 2012, 6(Suppl 4):O45http://www.biomedcentral.com/1753-6561/6/S4/O45© 2012 Phillips et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative CommonsAttribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction inany medium, provided the original work is properly cited.

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Development of an in vivo protocol for investigating natriuretic mechanisms in response to acute sodium loading in humans

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