A Chattopadhyay

A Neri

Andrew W. Horne

AW Horne

B Eskenazi

B Lindblom

B Pier

CM Farquhar

D Jurkovic

GB Campling

GF Assis

H Mai

Hazirah B. Z. Abidin

Hiroyoshi Ariga

Jeremy K. Brown

JL Shaw

Junko Nio-Kobayashi

Kai-Fai Lee

Lyndsey Boswell

M Jiang

O Gallo

P Jiang

PK Lala

RE Dickinson

RJ Stillman

RP Jansen

Stewart Burgess

T Magers

VN Sivalingam

W Hu

W. Colin Duncan

WC Duncan

WL Heusch

YL Lee

Z Jin

Zety E. H. A. Adin

PLoS ONE

PubMed

Horne Andrew W.

Brown Jeremy K.

Nio-Kobayashi Junko

Abidin Hazirah B. Z.

Adin Zety E. H. A.

Boswell Lyndsey

Burgess Stewart

Lee Kai-Fai

Duncan W. Colin

Public Library of Science (PLOS)

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube

Crossref

Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P&lt;0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P&lt;0.01) and increased levels of BCL2 transcript (P&lt;0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P&lt;0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy

Horne, Andrew W.

Brown, Jeremy K.

Nio-Kobayashi, Junko

Abidin, Hazirah B. Z.

Adin, Zety E. H. A.

Boswell, Lyndsey

Burgess, Stewart

Lee, Kai-fai

Duncan, W. Colin

Edinburgh Research Explorer

The Association between Smoking and Ectopic Pregnancy:Why Nicotine Is BAD for Your Fallopian Tube

Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P<0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy

Andrew W Horne

Jeremy K Brown

Hazirah B Z Abidin

Zety E H A Adin

W Colin Duncan

Directory of Open Access Journals

The association between smoking and ectopic pregnancy: why nicotine is BAD for your fallopian tube.

Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/ E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P, 0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy

Lee, Kai-Fai

Hokkaido University Collection of Scholarly and Academic Papers

English

Lee, CKF

Horne, AW

Brown, JK

Nio-Kobayashi, J

Abidin, HB

Adin, ZE

Boswell, L

Burgess, S

Duncan, WC

HKU Scholars Hub

The Association between Smoking and Ectopic Pregnancy: Why Nicotine Is BAD for Your Fallopian Tube.

<div>Epidemiological studies have shown that cigarette smoking is a major risk factor for tubal ectopic pregnancy but the reason for this remains unclear. Here, we set out to determine the effect of smoking on Fallopian tube gene expression. An oviductal epithelial cell line (OE-E6/E7) and explants of human Fallopian tubes from non-pregnant women (n = 6) were exposed to physiologically relevant concentrations of cotinine, the principle metabolite of nicotine, and changes in gene expression analyzed using the Illumina Human HT-12 array. Cotinine sensitive genes identified through this process were then localized and quantified in Fallopian tube biopsies from non-pregnant smokers (n = 10) and non-smokers (n = 11) using immunohistochemistry and TaqMan RT-PCR. The principle cotinine induced change in gene expression detected by the array analysis in both explants and the cell line was significant down regulation (P<0.05) of the pro-apoptotic gene BAD. We therefore assessed the effect of smoking on cell turnover in retrospectively collected human samples. Consistent with the array data, smoking was associated with decreased levels of BAD transcript (P<0.01) and increased levels of BCL2 transcript (P<0.05) in Fallopian tube biopsies. BAD and BCL2 specific immunolabelling was localized to Fallopian tube epithelium. Although no other significant differences in levels of apoptosis or cell cycle associated proteins were observed, smoking was associated with significant changes in the morphology of the Fallopian tube epithelium (P<0.05). These results suggest that smoking may alter tubal epithelial cell turnover and is associated with structural, as well as functional, changes that may contribute to the development of ectopic pregnancy.</div

Andrew W. Horne (209218)

Jeremy K. Brown (101879)

Junko Nio-Kobayashi (525504)

Hazirah B. Z. Abidin (525505)

Zety E. H. A. Adin (525506)

Lyndsey Boswell (525507)

Stewart Burgess (209199)

Kai-Fai Lee (195136)

W. Colin Duncan (209189)

The Francis Crick Institute

A functional role for nicotine in Bcl2 phosphorylation and suppression of apoptosis.

Analysis of epitopes on endometrial epithelium by scanning immunoelectron microscopy.

BAD/BCL-[X(L)] heterodimerization leads to bypass of G0/G1 arrest.

Bcl-2 constitutively suppresses p53-dependent apoptosis in colorectal cancer cells.

Bcl-2 overexpression and smoking history in head and neck cancer.

Cigarette smoke affects apoptosis in rat tongue mucosa: role of bcl-2 gene family.

Cigarette smoke inhalation affects the reproductive system of female hamsters.

Clinical implication of p53 mutation in lung cancer.

Contractile patterns of isolated oviductal smooth muscle under different hormonal conditions.

Cotinine exposure increases Fallopian tube PROKR1 expression via nicotinic AChRalpha-7: a potential mechanism explaining the link between smoking and tubal ectopic pregnancy.

Current knowledge of the aetiology of human tubal ectopic pregnancy.

Diagnosis and management of ectopic pregnancy.

Eckerling B

Ectopic pregnancy as a model to identify endometrial genes and signaling pathways important in decidualization and regulated by local trophoblast.

Ectopic pregnancy.

Effect of cigarette smoking on human oviductal ciliation and ciliogenesis.

Endocrine response in the fallopian tube.

Establishment and characterization of an immortalized human oviductal cell line.

Expression of the repulsive SLIT/ROBO pathway in the human endometrium and Fallopian tube.

Glucocorticoid regulation of SLIT/ROBO tumour suppressor genes in the ovarian surface epithelium and ovarian cancer cells.

Involvement of Bcl-2 family in apoptosis and signal pathways induced by cigarette smoke extract in the human airway smooth muscle cells.

Mechanisms of trophoblast invasiveness and their control: the role of proteases and protease inhibitors. Cancer Metastasis Rev 9: 369–379. Why Smoking Is BAD for Your Fallopian Tube

Nicotine induces multi-site phosphorylation of Bad in association with suppression of apoptosis.

Passive and active maternal smoking during pregnancy, as measured by serum continine, and postnatal smoke exposure. II. Effects on neurodeveloment at age 5 years.

Signalling pathways involved in nicotine regulation of apoptosis of human lung cancer cells.

Smoking and reproduction.

The Bad guy cooperates with good cop p53: Bad is transcriptionally up-regulated by p53 and forms a Bad/p53 complex at the mitochondria to induce apoptosis.

https://www.pure.ed.ac.uk/ws/files/13541195/The_Association_between_Smoking_and_Ectopic_Pregnancy_Why_Nicotine_Is_BAD_for_Your_Fallopian_Tube.pdf

The Association between Smoking and Ectopic Pregnancy:Why Nicotine Is BAD for Your Fallopian Tube

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