Browning GG

Felding JU

Frede S

Kania RE

Objective Ventilation of the chronically inflamed middle ear is a key outcome in functional middle ear surgery. Grommets eliminate middle ear effusion, but there is also evidence that they downregulate inflammation. The reason for this is not understood, but there is little to suggest alteration in Eustachian tube ventilatory capacity. Previous work has shown that the Junbo mouse model of chronic otitis media has hypoxic middle ear mucosa and bulla fluid leucocytes. Here we explore if surgical ventilation may alleviate chronic otitis media through downregulation of hypoxia. Study Design Surgical intervention on a mouse model of disease. Methods We established patency of myringotomy incision as 5 days in wild-type mice. We performed unilateral myringotomy on three cohorts of mice: 10 wild type controls, 12 Junbo mice, and 15 Junbo mice with additional removal of middle ear effusion. A small cohort of these mice were labeled in-vivo by intraperitoneal injection of pimodinazole to identify tissue hypoxia. Tissues were assessed for mucoperiosteal thickening and pimodinazole labeling, comparing operated to non-operated ears. Results Ventilation of the inflamed Junbo middle ear revealed significant reduction in inflammatory thickening associated with loss of pimodinazole labeling, suggesting resolution of cellular hypoxia. Conclusion Surgical ventilation may achieve therapeutic effect through alleviation of cellular hypoxia in the chronically inflamed middle ear. Targeted molecular therapy of hypoxia signaling may offer future alternative therapy for chronic OM

Bhutta, Mahmood F

Cheeseman, Michael T

Brown, Steve D M

Edinburgh Research Explorer

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

https://www.pure.ed.ac.uk/ws/files/15270099/Myringotomy_in_the_Junbo_mouse_model_of_chronic_otitis_media_alleviates_inflammation_and_cellular_hypoxia.pdf

Myringotomy in the Junbo mouse model of chronic otitis media alleviates inflammation and cellular hypoxia

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