This study aims to evaluate a reproductive systems augmented reality (AR) application for use in sexual health education. AR material included learner interactivity and a 3D video. Sixteen counselor candidates voluntarily registered to the sexual health class. A Reproductive Systems Questionnaire and an AR interview form were used to collect data. The participants were evaluated with a pretest, post-test and retention test. A significant improvement from pretest to post-test indicated that the AR application enhanced participants' knowledge of reproductive organs and their positions. Participants learned more about female reproductive systems than male reproductive systems