Abramoff

Azzopardi

Biswal

Bluth

Breedge Callaghan

Callaghan

Chen

Christopher A. Mitchell

Dhasaiyan

Hardin

Helen L. Lydon

Ibrahim M. Banat

Joshi-Navare

Niki Baccile

Piljac

Ribeiro

Roger Marchant

Saini

Sganga

Shah

Shao

Sleiman

Stipcevic

Turhan

Uchino

Van Bogaert

English

The sophorolipid class of biosurfactants are finding increasing use in personal care as well as pharmaceutical products and have the potential to disrupt biofilm formation and inhibit growth in a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from non-pathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast Starmerella bombicola, which yielded both acidic C 18-1 acetylated and non-acetylated congeners that were essentially free from other contaminants (&gt;95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents Enterococcus faecalis and Pseudomonas aeruginosa with significant reduction in c.f.u.'s at concentrations as low as 5 mgmL-1 In addition, the sophorolipid showed similar effects when combined with kanamycin or cefotaxime against the same two bacterial strains. As a potential use of these sophorolipids is as a component of topically applied creams for treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this we evaluated mammalian cell toxicity in vitro using viability tests and revealed no adverse effect on either endothelial or keratinocyte derived cell lines with sophorolipid concentration

Lydon, Helen

Baccile, Niki

Callaghan, Breedge

Marchant, R

Mitchell, CA

Banat, Ibrahim

Ulster University's Research Portal

Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing

ABSTRACT
          
            The sophorolipid class of biosurfactants is finding increasing use in personal care as well as pharmaceutical products and has the potential to disrupt biofilm formation and inhibit the growth of a variety of clinically relevant organisms. In order to investigate potential biomedical applications of sophorolipids derived from nonpathogenic organisms, we fractionated and purified glycolipid biosurfactant sophorolipids produced by the yeast
            Starmerella bombicola
            , which yielded nonacetylated acidic C
            18:1
            congeners that were essentially free from other contaminants (&gt;95% purity). These acidic sophorolipids have antimicrobial activities against the nosocomial infective agents
            Enterococcus faecalis
            and
            Pseudomonas aeruginosa
            , with significant reductions in CFU at concentrations of as low as 5 mg ml
            −1
            . In addition, the sophorolipid showed similar effects against the same two bacterial strains when combined with kanamycin or cefotaxime. As a potential use of these sophorolipids is as a component of topically applied creams for the treatment of wound infections, it is clear that they must have no demonstrable adverse effect on wound healing. To assess this, we evaluated mammalian cell toxicity
            in vitro
            using viability tests, which revealed no adverse effect on either endothelial or keratinocyte-derived cell lines with sophorolipid concentrations of &lt; 0.5 mg ml
            −1
            . In addition,
            in vivo
            experiments using a mouse skin wounding assay revealed that the time course of healing wounds was unaffected by the application of sophorolipid-containing creams, and histological examination of regenerated skin tissue confirmed that the healing process was similar to that observed for control animals, with no evidence of inflammation. These results are consistent with the suggestion that acidic sophorolipids can be used as a component of antimicrobial creams to reduce the risk of wound infection during healing.
          </jats:p

Crossref

Antimicrobial Agents and Chemotherapy

Adjuvant Antibiotic Activity of Acidic Sophorolipids with Potential for Facilitating Wound Healing

https://pure.ulster.ac.uk/ws/files/11604622/AAC02547-16.pdf

Adjuvant antibiotic activity of acidic sophorolipids with potential for facilitating wound healing

Abstract

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