Simple, precise, economical, fast and reliable two UV methods have been developed for the simultaneous estimation of Metformin HCl and Glimepiride in bulk and pharmaceutical dosage form. Method A is Absorbance maxima method, which is based on measurement of absorption at maximum wavelength of 236 nm and 228 nm for Metformin HCl and Glimepiride respectively. Method B is area under curve (AUC), in the wavelength range of 217-247 nm for Metformin HCl and 213-239 nm for Glimepiride. Linearity for detector response was observed in the concentration range of 5- 25?g/ml for Metformin HCl and 5-25 ?g/ml for Glimepiride. The accuracy of the methods was assessed by recovery studies and was found to be 100.23 % and 99.67 % for Metformin HCl and Glimepiride respectively. The developed method was validated with respect to linearity, accuracy (recovery), precision and specificity. The results were validated statistically as per ICH Q2 R1 guideline and were found to be satisfactory. The proposed methods were successfully applied for the determination of for Metformin HCl and Glimepiride in commercial pharmaceutical dosage form
