The aim of the present work is to develop simple, precise and economic UV- spectrophotometric methods for the simultaneous estimation of aspirin and omeprazole laboratory sample. The absorbance maxima (?max) for detection of aspirin and omeprazole were selected as 274 nm and 302 nm respectively for simultaneous equation method while wavelength range for detection of aspirin and omeprazole were selected as 270 nm - 276 nm and 300 nm - 305 nm respectively for area under curve method. Absorbance ratio method uses the ratio of absorbances at two selected wavelengths, one which is an isoabsorptive point and other being the ? max of one of the two components. From the overlay spectra of two drugs, it is evident that aspirin and omeprazole show an isoabsorptive point at 238.6 nm. Zero crossing first derivative spectrophotometry, where Aspirin showed zero crossing point at 301nm and Omeprazole showed zero crossing point at 274nm. Linearity for Aspirin was between 25- 125 ?g/mL and Omeprazole was 3-15 ?g/mL. These methods were successfully applied for estimation of Aspirin and Omeprazole in laboratory sample
