A simple, specific and accurate reverse phase high performance liquid chromatographic method was developed for simultaneous estimation of Cefixime and Dicloxacillin in bulk drug and tablet dosage form. Sepration was achieved by capcell pack C-18 column having 250 mm4.6 mm i.d. in isocratic mode, with mobile phase containing 0.05 M potassium dihydrogen phosphate: methanol (25:75), adjusted to pH 4.5 using ortho phosphoric acid. The flow rate was 1.0 ml/min and effluents were monitored at 232 nm. The retention time of Cefixime and Dicloxacillin were 3.19 min and 6.68 min respectively. The linearity for Cefixime and Dicloxacillin were in the range of 10-100 g/ml. The recoveries of Cefixime and Dicloxacillin were found in the range of 99.63-99.95 % and 99.58-99.98 % respectively. The proposed method was validated as per ICH and USP guidelines and successfully applied to the estimation of Cefixime and Dicloxacillin in bulk drug and tablet dosage form
