Henson, Sonal

Oyola, Samuel O.

English

CGSpace

Discovery of antigen-specific bovine antibodies through next-generation sequencingPOVERTY REDUCTION, LIVELIHOODS & JOBSPartnersUniversity of TorontoOutputs• Antibody repertoires of > 30 cows sequenced, including several from indigenous African breeds such as Ankole, Ndama and Boran.• Antibody repertoire characteristics of African breeds differ from those of exotic breeds.• Putative antigen-specific antibodies identified against an ECF vaccine candidate.• Novel alleles for immunoglobulin genes identified in African bovine breeds.The CGIAR Research Program on Livestock thanks all donors & organizations which globally support its work through their contributions to the CGIAR Trust Fund. cgiar.org/fundersThis document is licensed for use under the Creative Commons Attribution 4.0 International Licence. June 2020High throughput immune repertoire analysis by next-generation sequencing and bioinformatics provides qualitative and quantitative molecular information on antigen-specific responses.Context• To understand antibody responses to infection and vaccination so as to help design better vaccines.• Use antigen-specific antibodies to home in on critical components of antigens for use in vaccine development.• Add to the immunogenetic knowledge on African bovine breeds.• Developed the technology on East Coast Fever (ECF) experimental vaccine studies.Our innovative approach• To apply high throughput sequencing technology to characterize the quality of antibody response to immunization.• Developed a novel strategy to capture the unusually long antibodies found only in bovines that could have broadly neutralizing capacity.• Established pipeline for bioinformatics analysis on ILRI’s high performance computer.Future steps • Recombinant antigen-specific antibodies will be synthesized and tested for neutralizing activity.• This technology can be applied to study immune responses to other diseases of cattle, other ruminants as well as humans.• Antibody-based therapeutics can be developed from the sequence of protective antibodies.• Scaling is possible through automation of the analysis pipeline.Sonal Henson, ILRIs.henson@cgiar.orgSam Oyola, ILRIs.oyola@cgiar.orgLIVESTOCK HEALTH

Discovery of antigen-specific bovine antibodies through next-generation sequencing

https://cgspace.cgiar.org/bitstream/10568/109574/1/sonal.pdf

Discovery of antigen-specific bovine antibodies through next-generation sequencing

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