© Jabbar et al.; licensee BioMed Central Ltd. 2014
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The file attached is the Published/publisher’s pdf version of the article

Briscoe, AG

Foster, PG

Gasser, RB

Jabbar, A

Jex, AR

Littlewood, T

Mohandas, N

Müller, F

von Samson-Himmelstjerna, G

Parasites & Vectors

PubMed

© Jabbar et al.; licensee BioMed Central Ltd. 2014
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. The file attached is the Published/publisher’s pdf version of the article.NHM Repositor

Natural History Museum Repository

The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite

BACKGROUND: Parascaris univalens is an ascaridoid nematode of equids. Little is known about its epidemiology and population genetics in domestic and wild horse populations. PCR-based methods are suited to support studies in these areas, provided that reliable genetic markers are used. Recent studies have shown that mitochondrial (mt) genomic markers are applicable in such methods, but no such markers have been defined for P. univalens. METHODS: Mt genome regions were amplified from total genomic DNA isolated from P. univalens eggs by long-PCR and sequenced using Illumina technology. The mt genome was assembled and annotated using an established bioinformatic pipeline. Amino acid sequences inferred from all protein-encoding genes of the mt genomes were compared with those from other ascaridoid nematodes, and concatenated sequences were subjected to phylogenetic analysis by Bayesian inference. RESULTS: The circular mt genome was 13,920 bp in length and contained two ribosomal RNA, 12 protein-coding and 22 transfer RNA genes, consistent with those of other ascaridoids. Phylogenetic analysis of the concatenated amino acid sequence data for the 12 mt proteins showed that P. univalens was most closely related to Ascaris lumbricoides and A. suum, to the exclusion of other ascaridoids. CONCLUSIONS: This mt genome representing P. univalens now provides a rich source of genetic markers for future studies of the genetics and epidemiology of this parasite and its congener, P. equorum. This focus is significant, given that there is no published information on the specific prevalence and distribution of P. univalens infection in domestic and wild horse populations

Littlewood, DTJ

Von Samson-Himmelstjerna, G

University of Melbourne Institutional Repository

The mitochondrial genome of Parascaris univalens - Implications for a "forgotten" parasite

Parascaris univalens is an ascaridoid nematode of equids. Little is known
about its epidemiology and population genetics in domestic and wild horse
populations. PCR-based methods are suited to support studies in these areas,
provided that reliable genetic markers are used. Recent studies have shown
that mitochondrial (mt) genomic markers are applicable in such methods, but no
such markers have been defined for P. univalens. Mt genome regions were
amplified from total genomic DNA isolated from P. univalens eggs by long-PCR
and sequenced using Illumina technology. The mt genome was assembled and
annotated using an established bioinformatic pipeline. Amino acid sequences
inferred from all protein-encoding genes of the mt genomes were compared with
those from other ascaridoid nematodes, and concatenated sequences were
subjected to phylogenetic analysis by Bayesian inference. The circular mt
genome was 13,920 bp in length and contained two ribosomal RNA, 12 protein-
coding and 22 transfer RNA genes, consistent with those of other ascaridoids.
Phylogenetic analysis of the concatenated amino acid sequence data for the 12
mt proteins showed that P. univalens was most closely related to Ascaris
lumbricoides and A. suum, to the exclusion of other ascaridoids. This mt
genome representing P. univalens now provides a rich source of genetic markers
for future studies of the genetics and epidemiology of this parasite and its
congener, P. equorum. This focus is significant, given that there is no
published information on the specific prevalence and distribution of P.
univalens infection in domestic and wild horse populations

Jabbar, Abdul

Littlewood, D. Timothy J.

Mohandas, Namitha

Briscoe, Andrew G.

Foster, Peter G.

Müller, Fritz

Samson-Himmelstjerna, Georg von

Jex, Aaron R.

Gasser, Robin B.

Institutional Repository of the Freie Universität Berlin

The mitochondrial genome of Parascaris univalens - implications for a
“forgotten” parasite

Abdul Jabbar

D Timothy J Littlewood

Namitha Mohandas

Andrew G Briscoe

Peter G Foster

Fritz Müller

Georg von Samson-Himmelstjerna

Aaron R Jex

Robin B Gasser

Crossref

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The mitochondrial genome of Parascaris univalens - implications for a “forgotten” parasite

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Institutional Repository of the Freie Universität Berlin

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