© 2014 Rougerie et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  The attached file is the published version of the article

A Hausmann

A Riedel

AD Austin

Axel Hausmann

Ben J. Mans

BR Scheffers

D Lane

D Romero

DC Lees

DR Vieites

EJV Nieukerken

HCJ Godfray

I Letunic

Ian J. Kitching

J Beck

J Marin

JCE Riotte

JD Holloway

Jean Haxaire

JF Landry

JM Padial

JP Tuttle

K Tamura

M Kimura

M Mutanen

MA Smith

MI Krzywinski

MS Moulds

NP Kristensen

P Darge

P Sharma

Paul D. N. Hebert

PD Hebert

PDN Hebert

PM Oliver

PS Cranston

PS Gehring

PZ Goldstein

R Brechlin

R Rougerie

RM May

Rodolphe Rougerie

S Ratnasingham

S Wakamura

Scott E. Miller

SW James

T Vaglia

U Eitschberger

PLoS ONE

English

PubMed

International audienceMain Objective: We examine the extent of taxonomic and biogeographical uncertainty in a well-studied group of Australian Lepidoptera, the hawkmoths (Sphingidae). Methods: We analysed the diversity of Australian sphingids through the comparative analysis of their DNA barcodes, supplemented by morphological re-examinations and sequence information from a nuclear marker in selected cases. The results from the analysis of Australian sphingids were placed in a broader context by including conspecifics and closely related taxa from outside Australia to test taxonomic boundaries. Results: Our results led to the discovery of six new species in Australia, one case of erroneously synonymized species, and three cases of synonymy. As a result, we establish the occurrence of 75 species of hawkmoths on the continent. The analysis of records from outside Australia also challenges the validity of current taxonomic boundaries in as many as 18 species, including Agrius convolvuli (Linnaeus, 1758), a common species that has gained adoption as a model system. Our work has revealed a higher level of endemism than previously recognized. Most (90%) Australian sphingids are endemic to the continent (45%) or to Australia, the Pacific Islands and the Papuan and Wallacean regions (45%). Only seven species (10%) have ranges that extend beyond this major biogeographical boundary toward SE Asia and other regions of the Old World. Main Conclusions: This study has established that overlooked cryptic diversity and inaccurate species delineation produced significant misconceptions concerning diversity and distribution patterns in a group of insects that is considered well known taxonomically. Because DNA barcoding represents a straightforward way to test taxonomic boundaries, its implementation can improve the accuracy of primary diversity data in biogeography and conservation studies

Rougerie, Rodolphe

Kitching, Ian J.

Haxaire, Jean

Miller, Scott E.

Hausmann, Axel

Hebert, Paul D. N.

HAL: Hyper Article en Ligne

Australian Sphingidae – DNA Barcodes Challenge Current Species Boundaries and Distributions

<div>Main ObjectiveWe examine the extent of taxonomic and biogeographical uncertainty in a well-studied group of Australian Lepidoptera, the hawkmoths (Sphingidae).MethodsWe analysed the diversity of Australian sphingids through the comparative analysis of their DNA barcodes, supplemented by morphological re-examinations and sequence information from a nuclear marker in selected cases. The results from the analysis of Australian sphingids were placed in a broader context by including conspecifics and closely related taxa from outside Australia to test taxonomic boundaries.ResultsOur results led to the discovery of six new species in Australia, one case of erroneously synonymized species, and three cases of synonymy. As a result, we establish the occurrence of 75 species of hawkmoths on the continent. The analysis of records from outside Australia also challenges the validity of current taxonomic boundaries in as many as 18 species, including Agrius convolvuli (Linnaeus, 1758), a common species that has gained adoption as a model system. Our work has revealed a higher level of endemism than previously recognized. Most (90%) Australian sphingids are endemic to the continent (45%) or to Australia, the Pacific Islands and the Papuan and Wallacean regions (45%). Only seven species (10%) have ranges that extend beyond this major biogeographical boundary toward SE Asia and other regions of the Old World.Main ConclusionsThis study has established that overlooked cryptic diversity and inaccurate species delineation produced significant misconceptions concerning diversity and distribution patterns in a group of insects that is considered well known taxonomically. Because DNA barcoding represents a straightforward way to test taxonomic boundaries, its implementation can improve the accuracy of primary diversity data in biogeography and conservation studies.</div

Rodolphe Rougerie (134289)

Ian J. Kitching (263512)

Jean Haxaire (591527)

Scott E. Miller (167079)

Axel Hausmann (127091)

Paul D. N. Hebert (127093)

The Francis Crick Institute

Crossref

Main objectiveWe examine the extent of taxonomic and biogeographical uncertainty in a well-studied group of Australian Lepidoptera, the hawkmoths (Sphingidae).MethodsWe analysed the diversity of Australian sphingids through the comparative analysis of their DNA barcodes, supplemented by morphological re-examinations and sequence information from a nuclear marker in selected cases. The results from the analysis of Australian sphingids were placed in a broader context by including conspecifics and closely related taxa from outside Australia to test taxonomic boundaries.ResultsOur results led to the discovery of six new species in Australia, one case of erroneously synonymized species, and three cases of synonymy. As a result, we establish the occurrence of 75 species of hawkmoths on the continent. The analysis of records from outside Australia also challenges the validity of current taxonomic boundaries in as many as 18 species, including Agrius convolvuli (Linnaeus, 1758), a common species that has gained adoption as a model system. Our work has revealed a higher level of endemism than previously recognized. Most (90%) Australian sphingids are endemic to the continent (45%) or to Australia, the Pacific Islands and the Papuan and Wallacean regions (45%). Only seven species (10%) have ranges that extend beyond this major biogeographical boundary toward SE Asia and other regions of the Old World.Main conclusionsThis study has established that overlooked cryptic diversity and inaccurate species delineation produced significant misconceptions concerning diversity and distribution patterns in a group of insects that is considered well known taxonomically. Because DNA barcoding represents a straightforward way to test taxonomic boundaries, its implementation can improve the accuracy of primary diversity data in biogeography and conservation studies

Ian J Kitching

Scott E Miller

Paul D N Hebert

Directory of Open Access Journals

Australian Sphingidae--DNA barcodes challenge current species boundaries and distributions.

© 2014 Rougerie et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.  The attached file is the published version of the article.NHM Repositor

Rougerie, R

Haxaire, J

Miller, SE

Hausmann, A

Hebert, PDN

Kitching, I

Natural History Museum Repository

(Theme Report).

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A new species of Psilogramma

A review of the hawkmoth genus Hopliocnema Rothschild and Jordan, with the description of two new species (Lepidoptera: Sphingidae).

A simple method for estimating evolutionary rate of base substitution through comparative studies of nucleotide sequences.

Allopatry as a Gordian knot for taxonomists: patterns of DNA barcode divergence in Arctic-Alpine Lepidoptera.

Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species.

Basic biogeography: estimating biodiversity and mapping nature.

Biological identifications through DNA barcodes.

BOLD: The Barcode of Life Data System. Available: http://www.barcodinglife.org.

Challenge Australian Hawkmoths’ Taxonomy and Distribution

Circos: An information aesthetic for comparative genomics.

Cryptic diversity in vertebrates: molecular data double estimates of species diversity in a radiation of Australian lizards (Diplodactylus, Gekkota). Proc Roy Soc B-Biol

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DNA barcodes and morphology reveal a hybrid hawkmoth in Tahiti (Lepidoptera: Sphingidae).

DNA barcoding reveals a largely unknown fauna of Gracillariidae leaf mining moths in the Neotropics.

DNA barcoding reveals cryptic diversity

DNA Barcoding the geometrid fauna of Bavaria (Lepidoptera): successes, surprises, and questions.

DNA minibarcodes in taxonomic assignment: a morphologically unique new homoneurous moth clade from the Indian Himalayas described in Micropterix (Lepidoptera,

Einige Anmerkungen zur Gattung Psilogramma

Evolutionary framework for Lepidoptera model systems.

Four new species of the genus Psilogramma

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GenGIS 2: Geospatial analysis of traditional and genetic biodiversity, with new gradient algorithms and an extensible plugin framework.

Hawkmoths of the World. An annotated and illustrated revisionary checklist. Ithaca: The Natural History Museum, London &

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Hiding deep in the trees: discovery of divergent mitochondrial lineages in Malagasy chameleons of the Calumma nasutum group.

Insect biodiversity and conservation in Australasia.

Insects ‘Down Under’ - Diversity, endemism and evolution of the Australian insect fauna: examples from select orders.

Integrating DNA barcode data and taxonomic practice: determination, discovery, and description.

Integrative taxonomy on the fast track - towards more sustainability in biodiversity research.

Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation.

Lepidoptera phylogeny and systematics: the state of inventorying moth and butterfly diversity.

Local human-sweet potato-pig systems characterization and research

MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) Software Version 4.0.

Molecular approach to identify sibling species of the Ceriodaphnia cornuta (Cladocera: Daphniidae) from Australia with notes on the continental endemism of this group.

Morphology and molecules reveal unexpected cryptic diversity in the enigmatic genus Sinobirma Bryk,

Note sur Hippotion brennus (Lep.,

Numbers of living species in Australia and the world.

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Plutella australiana (Lepidoptera, Plutellidae), an overlooked diamondback moth revealed by DNA barcodes.

Revision der Gattung Psilogramma Rothschild

Revision of the Australian Oenochroma vinaria Guene´e, 1858 species-complex (Lepidoptera: Geometridae, Oenochrominae): DNA barcoding reveals cryptic diversity and assesses status of type specimen without dissection.

Sex pheromone of the sweetpotato hornworm, Agrius convolvuli (L.) (Lepidoptera: Sphingidae): identification of a major component and its activity in a wind tunnel.

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Vast underestimation of Madagascar’s biodiversity evidenced by an integrative amphibian inventory.

Wallace’s line revisited: has vicariance or dispersal shaped the distribution of Malesian hawkmoths (Lepidoptera: Sphingidae)?

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Wolbachia and DNA Barcoding Insects: Patterns, Potential, and Problems.

Zur Gattungszugehorigkeit von ‘‘Theretia’’ brunnea (Lep.: Sphingidae).

We examine the extent of taxonomic and biogeographical uncertainty in a well-studied group of Australian Lepidoptera, the hawkmoths (Sphingidae).We analysed the diversity of Australian sphingids through the comparative analysis of their DNA barcodes, supplemented by morphological re-examinations and sequence information from a nuclear marker in selected cases. The results from the analysis of Australian sphingids were placed in a broader context by including conspecifics and closely related taxa from outside Australia to test taxonomic boundaries. (Linnaeus, 1758), a common species that has gained adoption as a model system. Our work has revealed a higher level of endemism than previously recognized. Most (90%) Australian sphingids are endemic to the continent (45%) or to Australia, the Pacific Islands and the Papuan and Wallacean regions (45%). Only seven species (10%) have ranges that extend beyond this major biogeographical boundary toward SE Asia and other regions of the Old World.This study has established that overlooked cryptic diversity and inaccurate species delineation produced significant misconceptions concerning diversity and distribution patterns in a group of insects that is considered well known taxonomically. Because DNA barcoding represents a straightforward way to test taxonomic boundaries, its implementation can improve the accuracy of primary diversity data in biogeography and conservation studies

Rougerie Rodolphe

Kitching Ian J.

Haxaire Jean

Miller Scott E.

Hausmann Axel

Hebert Paul D. N.

Public Library of Science (PLOS)

Main Objective: We examine the extent of taxonomic and biogeographical uncertainty in a well-studied group of Australian Lepidoptera, the hawkmoths (Sphingidae).

Methods: We analysed the diversity of Australian sphingids through the comparative analysis of their DNA barcodes, supplemented by morphological re-examinations and sequence information from a nuclear marker in selected cases. The results from the analysis of Australian sphingids were placed in a broader context by including conspecifics and closely related taxa from outside Australia to test taxonomic boundaries.

Results: Our results led to the discovery of six new species in Australia, one case of erroneously synonymized species, and three cases of synonymy. As a result, we establish the occurrence of 75 species of hawkmoths on the continent. The analysis of records from outside Australia also challenges the validity of current taxonomic boundaries in as many as 18 species, including Agrius convolvuli (Linnaeus, 1758), a common species that has gained adoption as a model system. Our work has revealed a higher level of endemism than previously recognized. Most (90%) Australian sphingids are endemic to the continent (45%) or to Australia, the Pacific Islands and the Papuan and Wallacean regions (45%). Only seven species (10%) have ranges that extend beyond this major biogeographical boundary toward SE Asia and other regions of the Old World.

Main Conclusions: This study has established that overlooked cryptic diversity and inaccurate species delineation produced significant misconceptions concerning diversity and distribution patterns in a group of insects that is considered well known taxonomically. Because DNA barcoding represents a straightforward way to test taxonomic boundaries, its implementation can improve the accuracy of primary diversity data in biogeography and conservation studies

Australian Sphingidae – DNA Barcodes Challenge Current Species Boundaries and Distributions

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