F. Hernández y Juan F. Madrid. Universidad de Murcia. Departamento de Biología Celular e Histología
Abstract
In this study, we describe a new semiquantitative
method for measuring the extent of bleeding
in pathohistological tissue samples. To test our novel
method, we recruited 120 female patients in their first
trimester of pregnancy and divided them into three
groups of 40. Group I was the control group, in which no
dilation was applied. Group II was an experimental
group, in which dilation was performed using classical
mechanical dilators. Group III was also an experimental
group, in which dilation was performed using a
hydraulic dilator. Tissue samples were taken from the
patients’ cervical canals using a Novak’s probe via
energetic single-step curettage prior to any dilation in
Group I and after dilation in Groups II and III. After the
tissue samples were prepared, light microscopy was used
to obtain microphotographs at 100x magnification. The
surfaces affected by bleeding were measured in the
microphotographs using the Autodesk AutoCAD 2009
program and its “polylines” function. The lines were
used to mark the area around the entire sample (marked
A) and to create “polyline” areas around each bleeding
area on the sample (marked B). The percentage of the
total area affected by bleeding was calculated using the
formula: N=Bt x 100 / At where N is the percentage (%)
of the tissue sample surface affected by bleeding, At (A
total) is the sum of the surfaces of all of the tissue
samples and Bt (B total) is the sum of all the surfaces
affected by bleeding in all of the tissue samples. This
novel semi-quantitative method utilizes the Autodesk
AutoCAD 2009 program, which is simple to use and
widely available, thereby offering a new, objective and
precise approach to estimate the extent of bleeding in
tissue samples