A Ohkuni

A Shamseddine

AA Ejaeidi

AY Strongin

BL Lee

BS Nielsen

C Clarke

CM Perou

DE Misek

DJ Slamon

DT Liu

EA Perez

EC Keeley

F Tagliavini

GK Smyth

H Najjar

H Yanai

HJ Chang

J DeRisi

J Wang

JA Galvan

JJ Lee

JM Hunter

K Van Raemdonck

KB Chapman

KC Halsted

KM Bushby

KV Desai

L Chen

M Fang

M Hickey

M Maierthaler

M Yamamoto

MA Doumit

ML Nueda

MP Carrera

MT Weigel

MW Helms

N Bentzon

NA Lakkis

NS El Saghir

O Yersal

P Carmeliet

P Ortega

P Porter

P Zubor

PH Lo

R Ala-aho

RA Irizarry

S Basu

S Maubant

SK Gara

T Barrett

T Kawakami

T Nagahata

W Feng

Y Benjamini

Z Pei

ZK Stadler

Scientific Reports

PubMed

AbstractBreast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER®/Pathway Studio®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.</jats:p

Joelle Makoukji

Nadine J. Makhoul

Maya Khalil

Sally El-Sitt

Ehab Saad Aldin

Mark Jabbour

Fouad Boulos

Emanuela Gadaleta

Ajanthah Sangaralingam

Claude Chelala

Rose-Mary Boustany

Arafat Tfayli

Crossref

Gene expression profiling of breast cancer in Lebanese women

Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER ®/Pathway Studio ®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer. © 2016 The Author(s)

Makoukji, Joelle

Makhoul, Nadine J.

Khalil, Maya K.

El-Sitt, Sally Boulos

Saad Aldin, Ehab

Jabbour, Mark N.

Boulos, Fouad I.

Gadaleta, Emanuela

Sangaralingam, Ajanthah

Chelala, Claude

Boustany, Rose Mary Naaman

Tfayli, Arafat Hussein

AUB ScholarWorks (American Univ. of Beirut)

Makoukji, J

Makhoul, NJ

Khalil, M

El-Sitt, S

Aldin, ES

Jabbour, M

Boulos, F

Gadaleta, E

Sangaralingam, A

Chelala, C

Boustany, R-M

Tfayli, A

Queen Mary Research Online

https://qmro.qmul.ac.uk/xmlui/bitstream/123456789/31468/1/Chelala%20Gene%20expression%20profiling%20of%20breast%20cancer%20in%20Lebanese%20women%202016%20Published.pdf

Gene expression profiling of breast cancer in Lebanese women

Abstract

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Crossref

AUB ScholarWorks (American Univ. of Beirut)

Queen Mary Research Online