MRC-DTA studentship award 
British Pharmacological Society Integrative Awar

AC Carpentier

AC Nathwani

AM Maguire

AW Tong

AW Varley

C Ushida

C-Z Song

CA Brackley

CC Cunningham

D Ezer

DE Post

DL Viale

E Sharon

FA van de Loo

G Garaulet

GP Wang

H Hanawa

H Su

HB Gaspar

HM Prentice

I Barde

J Adriaansen

J Geurts

JE Butler

JK Raty

K Heyninck

K Takahashi

KC Dobi

KP Smith

L Giorgetti

L Guarente

L Henningsson

L Hu

L Wu

M Khoury

MC Subang

NJ Dusetti

OG Berg

Q Huang

S Cornelie

SE Halford

SH Kim

SL McKnight

T Shibata

V Gotea

V Matys

VJ Mutskov

WP Stemmer

YH Shin

Z Romero

Scientific Reports

PubMed

Mohamed, H

Chernajovsky, Y

Gould, D

Queen Mary Research Online

Assembly PCR synthesis of optimally designed, compact, multi-responsive promoters suited to gene therapy application

AbstractGene therapy has the potential to provide innovative treatments for genetic and non-genetic diseases, with the ability to auto-regulate expression levels of therapeutic molecules so that they are produced locally and in direct response to disease activity. Generating disease responsive gene therapy vectors requires knowledge of the activation profile of transcription factors (TFs) during active disease, in order to assemble binding sites for these TFs into synthetic promoters, which can be appropriately activated by the disease process. In this study, we optimised a PCR random assembly approach to generate promoters with optimal spacing between TF binding sites (TFBSs) and their distance from the TATA box. In promoters with optimal spacing, it was possible to demonstrate activation by individual transcription pathways and either additive or synergistic promoter activation when transfected cells were treated with combined stimuli. The kinetics and sensitivity of promoter activation was further explored in transduced cells and when lentivirus was directly delivered to mouse paws a synthetic promoter demonstrated excellent activation by real-time imaging in response to local inflammation.</jats:p

H. Mohamed

Y. Chernajovsky

D. Gould

Crossref

https://qmro.qmul.ac.uk/xmlui/bitstream/handle/123456789/19080/Gould%20Assembly%20PCR%20synthesis%202016%20Published_manuscript.pdf?sequence=2

Assembly PCR synthesis of optimally designed, compact, multi-responsive promoters suited to gene therapy application

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Queen Mary Research Online

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