This work was supported by project PI11/
01383 from Carlos III Institute of Health (General
Division of Evaluation and Research Promotion,
Madrid, Spain), which is integrated in National Plan of
Research, Development and Innovation (PN I+D+I
2008-2011). This project was cofinanced by
European Regional Development Fund (ERDF 2007-
2013

A Al-Ahmad

A Singh

AL Neely

BG Riep

C Hannig

CD Wu

CH Charles

CK Hope

DA Van Strydonck

David Suárez-Quintanilla

DH Fine

DN Tatakis

DR Korber

EM Decker

F Ulkur

G Pizzo

H Gu

H Jentsch

H Koo

H Loe

I Dige

I Tomás

Inmaculada Tomás

Isabel Prada-López

J Moran

J Pratten

JA Aas

JA Rodrigues

JE Stoeken

JW Costerton

Kimon Divaris

L García-Caballero

L Netuschil

M Rosin

MP Van Leeuwen

N Beyth

N Sharma

NB Arweiler

Nikolaos Donos

P Pan

PN Tawakoli

R Demke

RM Donlan

S Gandini

S Hahnel

SK Roberts

SR Wood

TM Auschill

Víctor Quintas

PLoS ONE

PubMed

Objective
To evaluate the in situ antiplaque effect after 4 days of using of 2 commercial antimicrobial
agents in short term on undisturbed plaque-like biofilm.
Trial Design and Participants
An observer-masked, crossover randomised clinical trial on 15 oral and systemically
healthy volunteers between 20–30 years who were randomly and sequentially allocated in
the same group which performed 3 interventions in different randomised sequences.
Intervention
The participants wore an appliance in 3 different rinsing periods doing mouthwashes twice
a day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At the
end of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning
Microscope.
Main Outcome Measures
Bacterial vitality, thickness and covering grade by the biofilm after 4 days of applying each
of the mouthwashes.
Results
The essential oils and the 0.2% chlorhexidine were significantly more effective than the sterile water at reducing bacterial vitality, thickness and covering grade by the biofilm. No significant differences were found between the 0.2% chlorhexidine and the essential oils at
reducing the bacterial vitality (13.2% vs. 14.7%). However, the 0.2% chlorhexidine showed
more reduction than the essential oils in thickness (6.5 μm vs. 10.0 μm; p<0.05) and covering grade by the biofilm (20.0% vs. 54.3%; p<0.001). Conclusion
The essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the
0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity.
Clinical Relevance
Daily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dental
plaque formation in the short term. Although 0.2% chlorhexidine continues to be the “gold
standard” in terms of antiplaque effect, essential oils could be considered a
reliable alternative.
Trial Registration
ClinicalTrials.gov NCT02124655This work was supported by project PI11/01383 from Carlos III Institute of Health (General Division of Evaluation and Research Promotion, Madrid, Spain), which is integrated in National Plan of Research, Development and Innovation (PN I+D+I 2008-2011). This project was cofinanced by European Regional Development Fund (ERDF 2007-2013)S

Quintas González, Víctor

Prada López, Isabel

Donos, Nikolaos

Suárez Quintanilla, David

Tomás Carmona, Inmaculada

Repositorio Institucional da Universidade de Santiago de Compostela

English

Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an In Situ Model of Oral Biofilm Growth: A Randomised Clinical Trial

antiplaque effect after 4 days of using of 2 commercial antimicrobial agents in short term on undisturbed plaque-like biofilm.An observer-masked, crossover randomised clinical trial on 15 oral and systemically healthy volunteers between 20–30 years who were randomly and sequentially allocated in the same group which performed 3 interventions in different randomised sequences.The participants wore an appliance in 3 different rinsing periods doing mouthwashes twice a day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At the end of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning Microscope.Bacterial vitality, thickness and covering grade by the biofilm after 4 days of applying each of the mouthwashes.. 54.3%; p<0.001).The essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the 0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity.Daily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dental plaque formation in the short term. Although 0.2% chlorhexidine continues to be the “gold standard” in terms of antiplaque effect, essential oils could be considered a reliable alternative

Quintas Víctor

Prada-López Isabel

Donos Nikolaos

Suárez-Quintanilla David

Tomás Inmaculada

Public Library of Science (PLOS)

 Model of Oral Biofilm Growth: A Randomised Clinical Trial

Crossref

<div>ObjectiveTo evaluate the in situ antiplaque effect after 4 days of using of 2 commercial antimicrobial agents in short term on undisturbed plaque-like biofilm.Trial Design and ParticipantsAn observer-masked, crossover randomised clinical trial on 15 oral and systemically healthy volunteers between 20–30 years who were randomly and sequentially allocated in the same group which performed 3 interventions in different randomised sequences.InterventionThe participants wore an appliance in 3 different rinsing periods doing mouthwashes twice a day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At the end of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning Microscope.Main Outcome MeasuresBacterial vitality, thickness and covering grade by the biofilm after 4 days of applying each of the mouthwashes.ResultsThe essential oils and the 0.2% chlorhexidine were significantly more effective than the sterile water at reducing bacterial vitality, thickness and covering grade by the biofilm. No significant differences were found between the 0.2% chlorhexidine and the essential oils at reducing the bacterial vitality (13.2% vs. 14.7%). However, the 0.2% chlorhexidine showed more reduction than the essential oils in thickness (6.5 μm vs. 10.0 μm; p<0.05) and covering grade by the biofilm (20.0% vs. 54.3%; p<0.001).ConclusionThe essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the 0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity.Clinical RelevanceDaily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dental plaque formation in the short term. Although 0.2% chlorhexidine continues to be the “gold standard” in terms of antiplaque effect, essential oils could be considered a reliable alternative.Trial RegistrationClinicalTrials.gov <a href="https://clinicaltrials.gov/ct2/show/NCT02124655" target="_blank">NCT02124655</a></div

Víctor Quintas (5662135)

Isabel Prada-López (502717)

Nikolaos Donos (692667)

David Suárez-Quintanilla (692668)

Inmaculada Tomás (385064)

The Francis Crick Institute

Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an In Situ Model of Oral Biofilm Growth: A Randomised Clinical Trial

Quintas, V

Prada-Lopez, I

Donos, N

Suarez-Quintanilla, D

Tomas, I

Queen Mary Research Online

OBJECTIVE:To evaluate the in situ antiplaque effect after 4 days of using of 2 commercial antimicrobial agents in short term on undisturbed plaque-like biofilm. TRIAL DESIGN AND PARTICIPANTS:An observer-masked, crossover randomised clinical trial on 15 oral and systemically healthy volunteers between 20-30 years who were randomly and sequentially allocated in the same group which performed 3 interventions in different randomised sequences. INTERVENTION:The participants wore an appliance in 3 different rinsing periods doing mouthwashes twice a day (1/0/1) with essential oils, 0.2% chlorhexidine or sterile water (negative control). At the end of each 4-day mouthwash period, samples were removed from the appliance. Posteriorly, after bacterial vital staining, samples were analysed using a Confocal Laser Scanning Microscope. MAIN OUTCOME MEASURES:Bacterial vitality, thickness and covering grade by the biofilm after 4 days of applying each of the mouthwashes. RESULTS:The essential oils and the 0.2% chlorhexidine were significantly more effective than the sterile water at reducing bacterial vitality, thickness and covering grade by the biofilm. No significant differences were found between the 0.2% chlorhexidine and the essential oils at reducing the bacterial vitality (13.2% vs. 14.7%). However, the 0.2% chlorhexidine showed more reduction than the essential oils in thickness (6.5 μm vs. 10.0 μm; p<0.05) and covering grade by the biofilm (20.0% vs. 54.3%; p<0.001). CONCLUSION:The essential oils and 0.2% chlorhexidine showed a high antiplaque effect. Although the 0.2% chlorhexidine showed better results with regard to reducing the thickness and covering grade by the biofilm, both antiseptics showed a high and similar antibacterial activity. CLINICAL RELEVANCE:Daily essential oils or 0.2% chlorhexidine mouthwashes are effective when reducing dental plaque formation in the short term. Although 0.2% chlorhexidine continues to be the "gold standard" in terms of antiplaque effect, essential oils could be considered a reliable alternative. TRIAL REGISTRATION:ClinicalTrials.gov NCT02124655

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Antiplaque effect of essential oils and 0.2% chlorhexidine on an in situ model of oral biofilm growth: a randomised clinical trial.

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Antiplaque Effect of Essential Oils and 0.2% Chlorhexidine on an In Situ Model of Oral Biofilm Growth: A Randomised Clinical Trial

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Repositorio Institucional da Universidade de Santiago de Compostela

Public Library of Science (PLOS)

Crossref

The Francis Crick Institute

Queen Mary Research Online

Directory of Open Access Journals