This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are creditedThis study was supported by the National Centre for the replacement, refinement and reduction of animals in research

A Paintner

AD Collier

AJ Roberts

AK Rose

B Adinoff

BD Kammerer

C Marchesi

C Rivier

Caroline H. Brennan

CB Kimmel

CH Brennan

CK Kim

E Park

FW Ellis

Gavin P. Vinson

GP Vinson

HN Richardson

IE de Jong

J Weinberg

JA Osborn

JF Lopez-Olmeda

JM Bailey

K Dedovic

KN Boyd

LJ Kily

LP Xia

LR Nelson

Matteo Baiamonte

MJ Reimers

ML Fuzzen

MM Przybycien-Szymanska

MO Parker

P Angelogianni

PA Keedwell

PV Piazza

R Spanagel

S Tran

SL Alderman

Stephan C.F. Neuhauss

Y Fernandes

Z Sarnyai

PLoS ONE

PubMed

), the aim is to determine whether developmental ethanol exposure provokes changes in the hypothalamo-pituitary-interrenal (HPI) axis (the teleost equivalent of the HPA), as it does in mammalian models, therefore opening the possibilities of using zebrafish to elucidate the mechanisms involved, and to test novel therapeutics to alleviate deleterious symptoms.The results showed that developmental exposure to ambient ethanol, 20mM-50mM 1-9 days post fertilisation, had immediate effects on the HPI, markedly reducing the cortisol response to air exposure stress, as measured by whole body cortisol content. This effect was sustained in adults 6 months later. Morphology, growth and locomotor activity of the animals were unaffected, suggesting a specific action of ethanol on the HPI. In this respect the data are consistent with mammalian results, although they contrast with the higher corticosteroid stress response reported in rats after developmental ethanol exposure. The mechanisms that underlie the specific sensitivity of the HPI to ethanol require elucidation

Baiamonte Matteo

Brennan Caroline H.

Vinson Gavin P.

Public Library of Science (PLOS)

Sustained Action of Developmental Ethanol Exposure on the Cortisol Response to Stress in Zebrafish Larvae and Adults

Crossref

BackgroundEthanol exposure during pregnancy is one of the leading causes of preventable birth defects, leading to a range of symptoms collectively known as fetal alcohol spectrum disorder. More moderate levels of prenatal ethanol exposure lead to a range of behavioural deficits including aggression, poor social interaction, poor cognitive performance and increased likelihood of addiction in later life. Current theories suggest that adaptation in the hypothalamo-pituitary-adrenal (HPA) axis and neuroendocrine systems contributes to mood alterations underlying behavioural deficits and vulnerability to addiction. In using zebrafish (Danio rerio), the aim is to determine whether developmental ethanol exposure provokes changes in the hypothalamo-pituitary-interrenal (HPI) axis (the teleost equivalent of the HPA), as it does in mammalian models, therefore opening the possibilities of using zebrafish to elucidate the mechanisms involved, and to test novel therapeutics to alleviate deleterious symptoms.Results and conclusionsThe results showed that developmental exposure to ambient ethanol, 20mM-50mM 1-9 days post fertilisation, had immediate effects on the HPI, markedly reducing the cortisol response to air exposure stress, as measured by whole body cortisol content. This effect was sustained in adults 6 months later. Morphology, growth and locomotor activity of the animals were unaffected, suggesting a specific action of ethanol on the HPI. In this respect the data are consistent with mammalian results, although they contrast with the higher corticosteroid stress response reported in rats after developmental ethanol exposure. The mechanisms that underlie the specific sensitivity of the HPI to ethanol require elucidation

Caroline H Brennan

Gavin P Vinson

Directory of Open Access Journals

Sustained action of developmental ethanol exposure on the cortisol response to stress in zebrafish larvae and adults.

Baiamonte, M

Brennan, CH

Vinson, GP

Queen Mary Research Online

file:///data/remote/core/dit/data/public_library_of_science/pdf/2fb/ZnRwOi8vZnRwLm5jYmkubmxtLm5paC5nb3YvcHViL3BtYy9vYV9wYWNrYWdlLzg2L2U4L1BNQzQzOTUyODgudGFyLmd6.pdf

Sustained Action of Developmental Ethanol Exposure on the Cortisol Response to Stress in Zebrafish Larvae and Adults

Abstract

Similar works

Full text

Available Versions

Public Library of Science (PLOS)

Crossref

Directory of Open Access Journals

Queen Mary Research Online

Public Library of Science (PLOS)