Data associated with the paper, "Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus.

Bradley-Stewart, Amanda

Cooper, Jonathan

Davis, Chris

Garrett, Alice

Gunson, Rory

Jajesniak, Pawel

Reboud, Julien

Sabir, Suleman R.

Thomson, Emma C.

Witkowska McConnell, Weronika

Xu, Gaolian

Yang, Zhugen

PubMed

The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in &lt;40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is manufactured at minimal cost using simple, portable equipment. Further development of the diagnostic test will facilitate linkage between disease diagnosis and treatment, greatly improving patient care pathways and reducing loss to follow-up, so assisting in the global elimination strategy

Cooper, Jonathan M.

Enlighten

Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus

Thomson, Emma

Enlighten: Research Data (University of Glasgow)

Loop mediated isothermal amplification as a powerful tool for early diagnosis of hepatitis C virus

LSHTM Data Compass

The early diagnosis of active hepatitis C virus (HCV) infection remains a significant barrier to the treatment of the disease and to preventing the associated significant morbidity and mortality seen, worldwide. Current testing is delayed due to the high cost, long turnaround times and high expertise needed in centralised diagnostic laboratories. Here we demonstrate a user-friendly, low-cost pan-genotypic assay, based upon reverse transcriptase loop mediated isothermal amplification (RT-LAMP). We developed a prototype device for point-of-care use, comprising a LAMP amplification chamber and lateral flow nucleic acid detection strips, giving a visually-read, user-friendly result in <40 min. The developed assay fulfils the current guidelines recommended by World Health Organisation and is manufactured at minimal cost using simple, portable equipment. Further development of the diagnostic test will facilitate linkage between disease diagnosis and treatment, greatly improving patient care pathways and reducing loss to follow-up, so assisting in the global elimination strategy

Sabir, Suleman R

Thomson, Emma C

Cooper, Jonathan M

LSHTM Research Online

Paper microfluidic implementation of loop mediated isothermal amplification for early diagnosis of hepatitis C virus.

http://eprints.gla.ac.uk/257804/1/257804.pdf

Loop mediated isothermal amplification as a powerful tool for early diagnosis of hepatitis C virus

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Enlighten

Enlighten: Research Data (University of Glasgow)

LSHTM Data Compass

LSHTM Research Online