Proteomics datasets, presented as a set of tables, produced as part of Dr Ryan Henrici's PhD project (completed 2018)

Sutherland, Colin J.

LSHTM Data Compass

 1  Protein ID Protein Name Average-Enrich. Triton Average-Enrich. CHAPS PF3D7_1147300 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_1459000 ATP-dependent RNA helicase DBP5 ∞ ND PF3D7_1251600 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_0720100 Small subunit rRNA processing protein, putative ∞ ND PF3D7_1123400 Translation elongation factor EF-1, subunit a ∞ ND PF3D7_1027400 DNA-directed RNA polymerase II subunit RPB7 ∞ ND PF3D7_0208700 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_1471400 Diacylglycerol kinase ∞ ND PF3D7_0611700 60S ribosomal protein L39 ∞ ND PF3D7_1138800 WD repeat-containing protein ∞ ∞ PF3D7_1434300 Hsp70/Hsp90 organizing protein ∞ ∞ PF3D7_1218300 AP-2 complex subunit mu 28.65 341.39 PF3D7_1114200 GTPase-activating protein, putative 21.83 ∞ PF3D7_0617100 AP-2 complex subunit alpha 21.01 240.76 PF3D7_1022600 Kelch protein K10 19.82 0.07 PF3D7_0217300 AP-2 complex subunit sigma 17.10 ∞ PF3D7_1461300 40S ribosomal protein S28e 9.75 ND PF3D7_0710400 DNA repair protein RAD14 8.44 ND PF3D7_0802000 Glutamate dehydrogenase 8.33 5.06 PF3D7_1137400 UVB-resistance protein UVR8 homologue 7.54 ND PF3D7_1205900 Conserved protein, unknown function 7.45 ∞ PF3D7_1402500 Ribosomal protein S27a 6.60 ∞ PF3D7_1470700 Conserved Plasmodium protein 6.60 ND PF3D7_1230900 Serine/threonine protein kinase RIO1 6.30 ∞ PF3D7_0508600 Conserved Plasmodium protein, unknown function 6.16 ND PF3D7_0321100 Conserved Plasmodium protein, unknown function 5.90 ND PF3D7_0813000 Conserved Plasmodium protein, unknown function 5.19 ND PF3D7_0801800 Mannose-6-phosphate isomerase 5.15 ND PF3D7_0529500 Cell cycle regulator protein 5.07 ND PF3D7_0212300 Peptide chain release factor subunit 1 4.93 ND PF3D7_0205800 PH domain-containing protein 4.74 ND PF3D7_0102200 Ring-infected erythrocyte surface antigen 4.66 ND PF3D7_1149200 Ring-infected erythrocyte surface antigen 3 4.62 ND PF3D7_0303300 DNA-directed RNA polymerases I, II, and III subunit RPABC2 4.45 ND PF3D7_0304200 EH domain-containing protein (EPS15) 4.43 ∞ PF3D7_1111000 tRNA m5C-methyltransferase 4.23 ND PF3D7_1109400 Essential nuclear protein 1 4.18 ND PF3D7_0106700 Small ribosomal subunit assembling AARP2 protein 4.18 ND PF3D7_1404500 rRNA biogenesis protein RRP5 4.09 ND PF3D7_1205700 Targeted glyoxalase II 3.90 ND  2    Supplementary Dataset 1A.  Extended IP-MS interactome of AP-2µ Factors identified with greater than 3-fold enrichment in HA pulldown in either condition compared to control pulldowns with statistical difference between HA and control pulldowns greater than 0 and at least 2 peptides per protein. ∞ indicates that no peptides were detected in the control pulldown; ND indicates protein was not identified in either the control or HA pulldown.    PF3D7_1104400 Thioredoxin 3.85 ND PF3D7_1451900 Ribosome biogenesis protein TSR1 3.83 ∞ PF3D7_0731300 Plasmodium exported protein (PHISTb), unknown function 3.83 ND PF3D7_0813600 Translation initiation factor SUI1 3.70 ND PF3D7_0416400 Histone acetyltransferase 3.70 ND PF3D7_0803100 U3 small nucleolar RNA-associated protein 14 3.65 ND PF3D7_1471000 RNA 3-terminal phosphate cyclase-like protein 3.61 ND PF3D7_0522300 18S rRNA (guanine-N(7))-methyltransferase 3.54 ND PF3D7_0708500 Heat shock protein 86 family protein 3.52 ND PF3D7_0528100 AP-1 complex subunit beta 3.49 26.11 PF3D7_1129100 Parasitophorous vacuolar protein 1 3.47 11.66 PF3D7_0722000 Conserved Plasmodium protein, unknown function 3.45 ∞ PF3D7_1010600 Eukaryotic translation initiation factor 2 subunit 3.41 ND PF3D7_0408500 Flap endonuclease 1 3.41 ∞ PF3D7_1108000 IWS1-like protein 3.39 ND PF3D7_0106000 Conserved Plasmodium protein 3.30 ND PF3D7_0417800 Cdc2-related protein kinase 1 3.19 ND PF3D7_0903400 ATP-dependent RNA helicase DDX60 3.16 ND PF3D7_1033300 Conserved Plasmodium protein, unknown function 3.09 ND PF3D7_0907600 Translation initiation factor SUI1 3.08 ND PF3D7_0205600 Conserved Plasmodium protein 3.04 ND PF3D7_1407500 Multifunctional methyltransferase subunit TRM 3.04 ∞ PF3D7_0926900 Replication termination factor 3.00 ND PF3D7_0615700 RNA export binding protein            1.73 ∞ PF3D7_1027300 peroxiredoxin  (oxidative stress defense) 1.63 159.73 PF3D7_1226900 Parasitophorous vacuolar protein 2 1.30 ∞ PF3D7_1323400 60S Ribosomal Protein L23 0.83 24.54 PF3D7_0312800 60S Ribosomal Protein L26 0.81 15.67 PF3D7_1222600 AP2 domain transcription factor AP2-G (nuclear) 0.53 27.51 PF3D7_1341300 60S Ribosomal Protein L18 0.49 15.35 PF3D7_1237200 Conserved Plasmodium protein, unknown function 0.46 10.11  3  Protein ID Protein Name Average-LFQ wt (N=3) Average-LFQ GFP (N=4) Enrichment -log(P) PF3D7_1421000 DIX domain-containing protein ND 9.42E+08 ∞ 3.34 PF3D7_1411300 DnaJ protein ND 7.14E+08 ∞ 5.05 PF3D7_0903400 ATP-dependent RNA helicase DDX60 ND 5.67E+08 ∞ 0.25 PF3D7_API0180 Apicoplast ribosomal protein L16 ND 1.30E+08 ∞ 0.62 PF3D7_0815800 Vacuolar protein sorting-associated protein Vps9  ND 1.25E+08 ∞ 2.26 PF3D7_1037600 TFIIH basal transcription factor complex helicase XPB subunit ND 7.32E+07 ∞ 0.27 PF3D7_0806800 V-type proton ATPase subunit A ND 6.37E+07 ∞ 2.09 PF3D7_0202600 Nucleic acid binding protein ND 6.27E+07 ∞ 0.40 PF3D7_1210100 Syntaxin, Qa-SNARE family ND 5.22E+07 ∞ 0.46 PF3D7_0907700 Proteasome activator 28 subunit beta ND 4.51E+07 ∞ 0.12 PF3D7_1458000 Cysteine proteinase falcipain 1 ND 3.84E+07 ∞ 0.64 PF3D7_0316500 Kinetochore protein NUF2 ND 3.15E+07 ∞ 0.01 PF3D7_0301700 Plasmodium exported protein, unknown function ND 3.12E+07 ∞ 0.49 PF3D7_0314100 Vesicle transport v-SNARE protein ND 2.95E+07 ∞ 0.60 PF3D7_1006700 Conserved Plasmodium protein, unknown function ND 2.94E+07 ∞ 0.06 PF3D7_1118100 AP-1 complex subunit sigma ND 2.90E+07 ∞ 0.15 PF3D7_0802400 Conserved Plasmodium protein, unknown function ND 2.89E+07 ∞ 0.00 PF3D7_0306100 Conserved Plasmodium protein, unknown function ND 2.79E+07 ∞ 0.21 PF3D7_0820500 Protein transport protein YIF1 ND 2.71E+07 ∞ 0.76 PF3D7_0320100 Protein transport protein SEC22 ND 2.51E+07 ∞ 0.02 PF3D7_1328100 Proteasome subunit beta type-7 ND 2.36E+07 ∞ 0.41 PF3D7_1110200 Pre-mRNA-processing factor 6 ND 2.21E+07 ∞ 0.02 PF3D7_0914900 BSD-domain protein ND 2.10E+07 ∞ 0.26 PF3D7_0103200 Nucleoside transporter 4 ND 1.89E+07 ∞ 0.48 PF3D7_0529000 Conserved Plasmodium protein, unknown function ND 1.86E+07 ∞ 0.62 PF3D7_0205500 DNA-directed RNA polymerase II 16 kDa subunit ND 1.86E+07 ∞ 0.03 PF3D7_1329500 Conserved  protein, unknown function ND 1.58E+07 ∞ 0.17 PF3D7_1464700 ATP synthase (C/AC39) subunit ND 1.23E+07 ∞ 0.15 PF3D7_1004400 RNA-binding protein ND 1.22E+07 ∞ 0.37 PF3D7_0931800 Proteasome subunit beta type-6 ND 9.31E+06 ∞ 0.07 PF3D7_1419400 Conserved Plasmodium membrane protein, unknown function ND 9.13E+06 ∞ 0.12 PF3D7_1435500 Clathrin light chain 7.17E+07 2.19E+09 30.56 2.74 PF3D7_1219100 Clathrin heavy chain 3.07E+09 6.87E+10 22.38 2.81 PF3D7_1307700 TOM1-like protein 3.32E+07 6.91E+08 20.81 2.78 PF3D7_1459600 Conserved Plasmodium protein, unknown function 1.17E+08 2.33E+09 19.89 2.31 PF3D7_1432800 HP12 protein homolog 1.72E+07 2.14E+08 12.40 1.63 PF3D7_1311400 AP-1 complex subunit mu-1 2.93E+07 3.18E+08 10.85 2.42 PF3D7_1353200 Membrane associated histidine-rich protein 5.61E+06 5.30E+07 9.45 0.35 PF3D7_1016300 GBP130 protein 1.75E+07 1.56E+08 8.92 2.38 PF3D7_1455500 AP-1 complex subunit gamma 7.43E+07 6.48E+08 8.72 2.27  4      Supplementary Dataset 1B.  Extended IP-MS interactome of clathrin heavy chain Factors identified with greater than 3-fold enrichment in GFP pulldowns compared to control pulldowns with statistical difference between GFP and control pulldowns greater than 0 and at least 2 peptides per protein. ∞ indicates that no peptides were detected in the control pulldown; ND indicates protein was not identified in either the control or HA pulldown.  PF3D7_1308200 Carbamoyl phosphate synthetase 2.60E+08 1.71E+09 6.57 3.07 PF3D7_1451800 Sortilin 6.96E+08 4.47E+09 6.42 1.51 PF3D7_1408700 Conserved protein, unknown function 3.24E+08 1.99E+09 6.15 1.33 PF3D7_1214900 Conserved protein, unknown function 2.96E+07 1.71E+08 5.78 1.93 PF3D7_0704400 Phosphoinositide-binding protein 4.90E+07 2.72E+08 5.55 1.58 PF3D7_0102200 Ring-infected erythrocyte surface antigen 5.42E+07 2.64E+08 4.87 2.16 PF3D7_0528100 AP-1 complex subunit beta, putative | tr 1.25E+08 5.69E+08 4.56 2.06 PF3D7_1215900 Serpentine receptor 2.48E+07 1.11E+08 4.47 1.27 PF3D7_0530100 SNARE protein 1.38E+07 5.95E+07 4.32 1.18 PF3D7_0936000 Ring-exported protein 2 1.76E+07 6.67E+07 3.80 0.90 PF3D7_0103900 Parasite-infected erythrocyte surface protein 2.84E+07 1.04E+08 3.65 1.28 PF3D7_0412000 LITAF-like zinc finger protein 2.06E+07 7.26E+07 3.52 0.36 PF3D7_1330400 ER lumen protein retaining receptor 1 1.01E+08 3.52E+08 3.50 1.26 PF3D7_0716300 Conserved protein, unknown function 1.03E+08 3.59E+08 3.47 1.36 PF3D7_1320000 Golgi protein 1 2.77E+07 9.49E+07 3.42 1.22 PF3D7_1359600 Conserved Plasmodium protein, unknown function 8.12E+08 2.77E+09 3.41 0.35 PF3D7_1015600 Heat shock protein 60 3.59E+07 1.22E+08 3.38 0.99 PF3D7_1010100 PI31 domain-containing protein 2.63E+07 8.86E+07 3.38 1.40 PF3D7_0532100 Early transcribed membrane protein 5 1.01E+08 3.36E+08 3.32 1.08 PF3D7_0936800 Plasmodium exported protein (PHISTc), unknown function  3.30E+07 1.08E+08 3.27 1.12 PF3D7_1123500 Golgi protein 2 2.97E+07 9.70E+07 3.26 1.10 PF3D7_0922100 Ubiquitin-like protein 2.32E+07 7.14E+07 3.07 0.17 PF3D7_1239700 ATP-dependent zinc metalloprotease FTSH 1 5.34E+07 1.60E+08 3.00 1.18 

The Plasmodium falciparum artemisinin susceptibility-associated AP-2 adaptin mu subunit is clathrin-independent and essential for schizont-maturation

http://datacompass.lshtm.ac.uk/1461/1/Proteomics_Tables_2019.pdf

The Plasmodium falciparum artemisinin susceptibility-associated AP-2 adaptin mu subunit is clathrin-independent and essential for schizont-maturation

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LSHTM Data Compass