Proteomics datasets, presented as a set of tables, produced as part of Dr Ryan Henrici's PhD project (completed 2018).  Version 2.0 represents an updated version as accepted for publication by mBio in January 2020

Henrici, Ryan

Sutherland, Colin

English

LSHTM Data Compass

 1  Protein ID Protein Name Average-Enrich. Triton Average-Enrich. CHAPS PF3D7_1147300 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_1459000 ATP-dependent RNA helicase DBP5 ∞ ND PF3D7_1251600 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_0720100 Small subunit rRNA processing protein, putative ∞ ND PF3D7_1123400 Translation elongation factor EF-1, subunit a ∞ ND PF3D7_1027400 DNA-directed RNA polymerase II subunit RPB7 ∞ ND PF3D7_0208700 Conserved Plasmodium protein, unknown function ∞ ND PF3D7_1471400 Diacylglycerol kinase ∞ ND PF3D7_0611700 60S ribosomal protein L39 ∞ ND PF3D7_1138800 WD repeat-containing protein ∞ ∞ PF3D7_1434300 Hsp70/Hsp90 organizing protein ∞ ∞ PF3D7_1218300 AP-2 complex subunit mu 28.65 341.39 PF3D7_1114200 GTPase-activating protein, putative 21.83 ∞ PF3D7_0617100 AP-2 complex subunit alpha 21.01 240.76 PF3D7_1022600 Kelch protein K10 19.82 0.07 PF3D7_0217300 AP-2 complex subunit sigma 17.10 ∞ PF3D7_1461300 40S ribosomal protein S28e 9.75 ND PF3D7_0710400 DNA repair protein RAD14 8.44 ND PF3D7_0802000 Glutamate dehydrogenase 8.33 5.06 PF3D7_1137400 UVB-resistance protein UVR8 homologue 7.54 ND PF3D7_1205900 Conserved protein, unknown function 7.45 ∞ PF3D7_1402500 Ribosomal protein S27a 6.60 ∞ PF3D7_1470700 Conserved Plasmodium protein 6.60 ND PF3D7_1230900 Serine/threonine protein kinase RIO1 6.30 ∞ PF3D7_0508600 Conserved Plasmodium protein, unknown function 6.16 ND PF3D7_0321100 Conserved Plasmodium protein, unknown function 5.90 ND PF3D7_0813000 KIC7 5.19 ND PF3D7_0801800 Mannose-6-phosphate isomerase 5.15 ND PF3D7_0529500 Cell cycle regulator protein 5.07 ND PF3D7_0212300 Peptide chain release factor subunit 1 4.93 ND PF3D7_0205800 PH domain-containing protein 4.74 ND PF3D7_0102200 Ring-infected erythrocyte surface antigen 4.66 ND PF3D7_1149200 Ring-infected erythrocyte surface antigen 3 4.62 ND PF3D7_0303300 DNA-directed RNA polymerases I, II, and III subunit RPABC2 4.45 ND PF3D7_0304200 EH domain-containing protein (EPS15) 4.43 ∞ PF3D7_1111000 tRNA m5C-methyltransferase 4.23 ND PF3D7_1109400 Essential nuclear protein 1 4.18 ND PF3D7_0106700 Small ribosomal subunit assembling AARP2 protein 4.18 ND PF3D7_1404500 rRNA biogenesis protein RRP5 4.09 ND PF3D7_1205700 Targeted glyoxalase II 3.90 ND  2    Supplementary Dataset 1A.  Extended IP-MS interactome of AP-2µ Factors identified with greater than 3-fold enrichment in HA pulldown in either condition compared to control pulldowns with statistical difference between HA and control pulldowns greater than 0 and at least 2 peptides per protein. ∞ indicates that no peptides were detected in the control pulldown; ND indicates protein was not identified in either the control or HA pulldown.    PF3D7_1104400 Thioredoxin 3.85 ND PF3D7_1451900 Ribosome biogenesis protein TSR1 3.83 ∞ PF3D7_0731300 Plasmodium exported protein (PHISTb), unknown function 3.83 ND PF3D7_0813600 Translation initiation factor SUI1 3.70 ND PF3D7_0416400 Histone acetyltransferase 3.70 ND PF3D7_0803100 U3 small nucleolar RNA-associated protein 14 3.65 ND PF3D7_1471000 RNA 3-terminal phosphate cyclase-like protein 3.61 ND PF3D7_0522300 18S rRNA (guanine-N(7))-methyltransferase 3.54 ND PF3D7_0708500 Heat shock protein 86 family protein 3.52 ND PF3D7_0528100 AP-1 complex subunit beta 3.49 26.11 PF3D7_1129100 Parasitophorous vacuolar protein 1 3.47 11.66 PF3D7_0722000 Conserved Plasmodium protein, unknown function 3.45 ∞ PF3D7_1010600 Eukaryotic translation initiation factor 2 subunit 3.41 ND PF3D7_0408500 Flap endonuclease 1 3.41 ∞ PF3D7_1108000 IWS1-like protein 3.39 ND PF3D7_0106000 Conserved Plasmodium protein 3.30 ND PF3D7_0417800 Cdc2-related protein kinase 1 3.19 ND PF3D7_0903400 ATP-dependent RNA helicase DDX60 3.16 ND PF3D7_1033300 Conserved Plasmodium protein, unknown function 3.09 ND PF3D7_0907600 Translation initiation factor SUI1 3.08 ND PF3D7_0205600 Conserved Plasmodium protein 3.04 ND PF3D7_1407500 Multifunctional methyltransferase subunit TRM 3.04 ∞ PF3D7_0926900 Replication termination factor 3.00 ND PF3D7_0615700 RNA export binding protein            1.73 ∞ PF3D7_1027300 peroxiredoxin  (oxidative stress defense) 1.63 159.73 PF3D7_1226900 Parasitophorous vacuolar protein 2 1.30 ∞ PF3D7_1323400 60S Ribosomal Protein L23 0.83 24.54 PF3D7_0312800 60S Ribosomal Protein L26 0.81 15.67 PF3D7_1222600 AP2 domain transcription factor AP2-G (nuclear) 0.53 27.51 PF3D7_1341300 60S Ribosomal Protein L18 0.49 15.35 PF3D7_1237200 Conserved Plasmodium protein, unknown function 0.46 10.11  3  Protein ID Protein Name Average-LFQ wt (N=3) Average-LFQ GFP (N=4) Enrichment -log(P) PF3D7_1421000 DIX domain-containing protein ND 9.42E+08 ∞ 3.34 PF3D7_1411300 DnaJ protein ND 7.14E+08 ∞ 5.05 PF3D7_0903400 ATP-dependent RNA helicase DDX60 ND 5.67E+08 ∞ 0.25 PF3D7_API0180 Apicoplast ribosomal protein L16 ND 1.30E+08 ∞ 0.62 PF3D7_0815800 Vacuolar protein sorting-associated protein Vps9  ND 1.25E+08 ∞ 2.26 PF3D7_1037600 TFIIH basal transcription factor complex helicase XPB subunit ND 7.32E+07 ∞ 0.27 PF3D7_0806800 V-type proton ATPase subunit A ND 6.37E+07 ∞ 2.09 PF3D7_0202600 Nucleic acid binding protein ND 6.27E+07 ∞ 0.40 PF3D7_1210100 Syntaxin, Qa-SNARE family ND 5.22E+07 ∞ 0.46 PF3D7_0907700 Proteasome activator 28 subunit beta ND 4.51E+07 ∞ 0.12 PF3D7_1458000 Cysteine proteinase falcipain 1 ND 3.84E+07 ∞ 0.64 PF3D7_0316500 Kinetochore protein NUF2 ND 3.15E+07 ∞ 0.01 PF3D7_0301700 Plasmodium exported protein, unknown function ND 3.12E+07 ∞ 0.49 PF3D7_0314100 Vesicle transport v-SNARE protein ND 2.95E+07 ∞ 0.60 PF3D7_1006700 Conserved Plasmodium protein, unknown function ND 2.94E+07 ∞ 0.06 PF3D7_1118100 AP-1 complex subunit sigma ND 2.90E+07 ∞ 0.15 PF3D7_0802400 Conserved Plasmodium protein, unknown function ND 2.89E+07 ∞ 0.00 PF3D7_0306100 Conserved Plasmodium protein, unknown function ND 2.79E+07 ∞ 0.21 PF3D7_0820500 Protein transport protein YIF1 ND 2.71E+07 ∞ 0.76 PF3D7_0320100 Protein transport protein SEC22 ND 2.51E+07 ∞ 0.02 PF3D7_1328100 Proteasome subunit beta type-7 ND 2.36E+07 ∞ 0.41 PF3D7_1110200 Pre-mRNA-processing factor 6 ND 2.21E+07 ∞ 0.02 PF3D7_0914900 BSD-domain protein ND 2.10E+07 ∞ 0.26 PF3D7_0103200 Nucleoside transporter 4 ND 1.89E+07 ∞ 0.48 PF3D7_0529000 Conserved Plasmodium protein, unknown function ND 1.86E+07 ∞ 0.62 PF3D7_0205500 DNA-directed RNA polymerase II 16 kDa subunit ND 1.86E+07 ∞ 0.03 PF3D7_1329500 Conserved  protein, unknown function ND 1.58E+07 ∞ 0.17 PF3D7_1464700 ATP synthase (C/AC39) subunit ND 1.23E+07 ∞ 0.15 PF3D7_1004400 RNA-binding protein ND 1.22E+07 ∞ 0.37 PF3D7_0931800 Proteasome subunit beta type-6 ND 9.31E+06 ∞ 0.07 PF3D7_1419400 Conserved Plasmodium membrane protein, unknown function ND 9.13E+06 ∞ 0.12 PF3D7_1435500 Clathrin light chain 7.17E+07 2.19E+09 30.56 2.74 PF3D7_1219100 Clathrin heavy chain 3.07E+09 6.87E+10 22.38 2.81 PF3D7_1307700 TOM1-like protein 3.32E+07 6.91E+08 20.81 2.78 PF3D7_1459600 Conserved Plasmodium protein, unknown function 1.17E+08 2.33E+09 19.89 2.31 PF3D7_1432800 HP12 protein homolog 1.72E+07 2.14E+08 12.40 1.63 PF3D7_1311400 AP-1 complex subunit mu-1 2.93E+07 3.18E+08 10.85 2.42 PF3D7_1353200 Membrane associated histidine-rich protein 5.61E+06 5.30E+07 9.45 0.35 PF3D7_1016300 GBP130 protein 1.75E+07 1.56E+08 8.92 2.38 PF3D7_1455500 AP-1 complex subunit gamma 7.43E+07 6.48E+08 8.72 2.27  4      Supplementary Dataset 1B.  Extended IP-MS interactome of clathrin heavy chain Factors identified with greater than 3-fold enrichment in GFP pulldowns compared to control pulldowns with statistical difference between GFP and control pulldowns greater than 0 and at least 2 peptides per protein. ∞ indicates that no peptides were detected in the control pulldown; ND indicates protein was not identified in either the control or HA pulldown.  PF3D7_1308200 Carbamoyl phosphate synthetase 2.60E+08 1.71E+09 6.57 3.07 PF3D7_1451800 Sortilin 6.96E+08 4.47E+09 6.42 1.51 PF3D7_1408700 Conserved protein, unknown function 3.24E+08 1.99E+09 6.15 1.33 PF3D7_1214900 Conserved protein, unknown function 2.96E+07 1.71E+08 5.78 1.93 PF3D7_0704400 Phosphoinositide-binding protein 4.90E+07 2.72E+08 5.55 1.58 PF3D7_0102200 Ring-infected erythrocyte surface antigen 5.42E+07 2.64E+08 4.87 2.16 PF3D7_0528100 AP-1 complex subunit beta, putative | tr 1.25E+08 5.69E+08 4.56 2.06 PF3D7_1215900 Serpentine receptor 2.48E+07 1.11E+08 4.47 1.27 PF3D7_0530100 SNARE protein 1.38E+07 5.95E+07 4.32 1.18 PF3D7_0936000 Ring-exported protein 2 1.76E+07 6.67E+07 3.80 0.90 PF3D7_0103900 Parasite-infected erythrocyte surface protein 2.84E+07 1.04E+08 3.65 1.28 PF3D7_0412000 LITAF-like zinc finger protein 2.06E+07 7.26E+07 3.52 0.36 PF3D7_1330400 ER lumen protein retaining receptor 1 1.01E+08 3.52E+08 3.50 1.26 PF3D7_0716300 Conserved protein, unknown function 1.03E+08 3.59E+08 3.47 1.36 PF3D7_1320000 Golgi protein 1 2.77E+07 9.49E+07 3.42 1.22 PF3D7_1359600 Conserved Plasmodium protein, unknown function 8.12E+08 2.77E+09 3.41 0.35 PF3D7_1015600 Heat shock protein 60 3.59E+07 1.22E+08 3.38 0.99 PF3D7_1010100 PI31 domain-containing protein 2.63E+07 8.86E+07 3.38 1.40 PF3D7_0532100 Early transcribed membrane protein 5 1.01E+08 3.36E+08 3.32 1.08 PF3D7_0936800 Plasmodium exported protein (PHISTc), unknown function  3.30E+07 1.08E+08 3.27 1.12 PF3D7_1123500 Golgi protein 2 2.97E+07 9.70E+07 3.26 1.10 PF3D7_0922100 Ubiquitin-like protein 2.32E+07 7.14E+07 3.07 0.17 PF3D7_1239700 ATP-dependent zinc metalloprotease FTSH 1 5.34E+07 1.60E+08 3.00 1.18 

The Plasmodium falciparum artemisinin susceptibility-associated AP-2 adaptin mu subunit is clathrin-independent and essential for schizont-maturation v2.0

http://datacompass.lshtm.ac.uk/1533/1/Henrici_et_al_Proteomics_Tables_2019_v2.0.pdf

The Plasmodium falciparum artemisinin susceptibility-associated AP-2 adaptin mu subunit is clathrin-independent and essential for schizont-maturation v2.0

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