Acidophiles Oren A

Ayako Kurihara

Blocher JC

Chisato Suwa

Daisuke Mitsuya

Masahiko Okai

Masami Ishida

McDonald LC

Naoto Urano

Nobuo Obara

Shintaro Nagaoka

Abstract
               We isolated Cryptococcus sp. T1 from Lake Tazawa’s acidic water in Japan. Cryptococcus sp. T1 neutralized an acidic casamino acid solution (pH 3.0) and released ammonia from the casamino acids to aid the neutralization. The neutralization volume was estimated to be approximately 0.4 mL/h. The casamino acids’ amino acids decreased (1.24→0.15 mM); ammonia increased (0.22→0.99 mM). We neutralized acidic drainage water (1 L) from a Tamagawa River neutralization plant, which was run through the column with the T1-immobilized alginate beads at a flow rate of 0.5 mL/min, and observed that the viscosity, particle size and amounts of the alginate beads affected the acidic drainage neutralization with an increase of the pH value from 5.26 to 6.61 in the last fraction. An increase in the Al concentration decreased Cryptococcus sp. T1’s neutralization ability. After 48 h, the pH of acidic water with 50 mg/L Al was apparently lower than that without Al. Almost no pH increase was observed at 75 mg/L.</jats:p

Crossref

Bioscience Biotechnology and Biochemistry

Neutralization of acidic drainage by <i>Cryptococcus</i> sp. T1 immobilized in alginate beads

Neutralization of acidic drainage by
                    Cryptococcus

Neutralization of acidic drainage by Cryptococcus

Abstract

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