CORE
🇺🇦
make metadata, not war
Services
Services overview
Explore all CORE services
Access to raw data
API
Dataset
FastSync
Content discovery
Recommender
Discovery
OAI identifiers
OAI Resolver
Managing content
Dashboard
Bespoke contracts
Consultancy services
Support us
Support us
Membership
Sponsorship
Community governance
Advisory Board
Board of supporters
Research network
About
About us
Our mission
Team
Blog
FAQs
Contact us
research
Lactobacillus casei strain Shirota selectively modulates macrophage subset cytokine production
Authors
J Beal
AD Foey
N Habil
Publication date
1 December 2012
Publisher
Abstract
Probiotics confer health benefits through many mechanisms including modulation of the gut immune system, dut macrophages regulate immune homeostasis, mounting tolerogenic responses to food and commensal bacteria or immune inflammatory responses to pathogens. Local environment and macrophage subset determine immune response and tolerance, associated with an M2-like phenotype and inflammatory activation with an Ml-like phenotype. Subset predominance will determine immunomodulatory effects of probiotic species such as Lactobacillus casei strain Shirota (LcS). The aim of this study was to investigate differential regulatory effects of LcS on Ml and M2 macrophage subsets. PMA or vitamin D3 differentiated THP-1 human monocytic cells were used to investigate heat-killed LcS and secreted protein tmmunoregulation of Ml and M2 cytokine production, respectively. Additionally, regulation of CD 14lo M2 and CD 14hiMl function was investigated Cytokine expression was measured by ELISA and NFkB activity by reporter assay. Both HK-LcS and SP-LcSaugmented IL-lβ, suppressed IL-6 and differentially regulated TNFα and IL-8, dependent on macrophage subset. HK-LcS and LcS-SP augmented CD14hi Ml TNFa whereas suppressed CD14lo M2 IL-6 and CD14hi Ml NFkB. In conclusion, LcS differentially regulates macrophage cytokines and NFkB activation, is subset-dependent and suggests a cautionary approach to probiotic treatment of mucosal inflammation. Copyright © 2012 by New Century Health Publishers, LLC
Similar works
Full text
Open in the Core reader
Download PDF
Available Versions
Plymouth Electronic Archive and Research Library
See this paper in CORE
Go to the repository landing page
Download from data provider
oai:pearl.plymouth.ac.uk:10026...
Last time updated on 31/10/2018