Sucralose (SUC) is an artificial sweetener that is now widely used in North American and Europe; it has been de- tected in a wide variety of aquatic environments. It is considered safe for human consumption but its effects in the ecosystem have not yet been studied in depth, since limited ecotoxicological data are available in the peer- reviewed literature. This study aimed to evaluate potential SUC-induced toxicological hazard in the blood, brain, gill, liver and muscle of Cyprinus carpio using oxidative stress biomarkers. Carps were exposed to two dif- ferent environmentally relevant concentrations (0.05 and 155 μg L− 1) for different exposure times (12, 24, 48, 72 and 96 h). The following biomarkers were evaluated: lipid peroxidation (LPX), hydroperoxide content (HPC) and protein carbonyl content (PCC), as well as the activity of antioxidant enzymes, superoxide dismutase (SOD) and catalase (CAT). SUC was determined by high pressure liquid chromatography tandem mass spectrometry tech- niques (HPLC)–MS/MS. Results show a statically significant increase in LPX, HPC, PCC (P b 0.05) especially in gill, brain and muscle, as well as significant changes in the activity of antioxidant enzymes in gill and muscle. Fur- thermore, the biomarkers employed in this study are useful in the assessment of the environmental impact of this agent on aquatic species