Development of artificial systems to mimic cellular structures and functions

Abstract

Membrane models proved its uniqueness as a tool for biophysics studies at single molecule level. Despite impressive progress in the field of biomimetic membrane models, there are serious challenges limiting their wider use in biological application, for example their stability and ability for communication with different environment. This thesis described the development and uses of artificial membrane for biophysical applications. The initial focus was on the investigation and design of a new membrane model platform that mimics biological cell and tissues. In the first study we built a model membrane platform by encapsulating one of membrane model called Droplet Interface Bilayer in hydrogel and we test the function ability of this system by incorporating pore forming proteins. In the second study we characterize amyloid peptides oligomers behavior using planar lipid technique. In the final study of this thesis we designed and construct membrane nanomachine from DNA which it produce nanomechanical motion and transport across the membranestatus: publishe