Studium produkce lakázy vybranými druhy hub

Abstract

The importance of lignin-degrading fungi and their ligninolytic enzymes has been well appreciated globally, because of their potential use in various industries. This bachelor thesis is focused on study of potential production of extracellular ligninolytic enzymes by brown rot fungi. In the literary review selected strains of brown rot fungi and its most studied ligninolytic enzymes are characterized. The use of laccase in various industries especially food industry are summarized. Activity of ligninolytic enzymes produced by brown rot fungi Laetiporus sulphureus, Serpula lacrymans, Gloeophyllum sepiarium, Phaeolus schweinitzii and Laetiporus montanus during submerged cultivation for 24 days was evaluated. Laccase, lignin peroxidase and manganese dependent peroxidase activities were measured spectrophotometrically. For all enzymes, the highest activity was observed on the 12 day. Selected fungi produced the most lignin peroxidase. Laetiporus sulphureus and Gloeophyllum sepiarium showed the highest values of enzyme activities. It has been found that the enzymatic activity of fungi depends on the geographical location and the specific habitat. Enzyme activities of the same strain taken from different locations were different