Maize (Zea mays L.) is the third most crucial crop worldwide and is of paramount importance in both humansand livestock diets. Conventional maize varieties have less than half of the amino acids recommended for humannutrition, and this deficiency results in an imbalance of amino acids and low protein content, which has been associatedwith several pathologies, including malnutrition. Thus, different countries have focused on research onfortified foods, such as quality protein maize (QPM) noting that these improved varieties may contain up to 100%more essential amino acids residues than conventional maize. Hence, this study aimed to characterize throughtandem mass spectrometry and bioinformatics analysis, relative expression of polypeptides contained in a hybridvariety of QPM, which allow to identify potential markers with implications in the management and improvementof this crops maintaining their intrinsic characteristics. We identify 262 polypeptides, highlighting those related tomolecular function (catalytic activity, structural molecule activity, and binding) and biological process (cellular andmetabolic process). These results provide the necessary information, not only for the characterization of the QPMproteome through novel tools such as proteomics, but also to describe mechanisms related to different biologicalprocesses such as the embryogenesis, development and growth of grains and eventually plants. PotentiallyIt promotes the discovery of molecular markers (biomarkers) that would allow the improvement of agronomicalprocesses