Buckle, R. S.

Marsh, P. J.

Snyder, D.

Zhelev, N. Zh

Light scattering experiments were undertaken to study binding of the Max transcription factor to its E-box DNA recognition sequence. Translational diffusion coefficients were measured and the average hydrodynamic radii (Rh) of complexes calculated using the Stokes-Einstein equation. We detected both dimerization of Max and the formation of a stable complex with its E-box DNA target. These results demonstrate the applicability of Dynamic Light Scattering for measuring protein-DNA interactions.</p

Studying protein-DNA binding by dynamic light scattering

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University of Dundee Online Publications

University of Dundee Online Publications