Allcock

Andrews

Callis

Chen

Dos Remedios

Juzeliunas

Kierdaszuk

Lakowicz

Maiti

English

Fluorescence resonance energy transfer (FRET) is a technique now widely applied to probe biological and other complex systems for the determination of fluorophore separation and structure. Recently the theory behind the anisotropy of fluorescence has been extended to include the case of a residual polarization following energy transfer between fluorophores, and here the theory is further extended to accommodate two-photon excitation. This reveals not only novel polarization characteristics but also a distance dependence whose analysis does not require a priori knowledge of the donor–acceptor spectral overlap. The two-photon FRET anisotropy results mirror their one-photon counterparts, in terms of fluorophore separation characteristics and also their relationship to the anisotropies for isolated fluorophores. Moreover, the two-photon results are not restricted to a plane polarized input, results being given for both plane and circularly polarized pump radiation

Allcock, Philip

Andrews, David L.

University of East Anglia digital repository

Two-photon fluorescence: Resonance energy transfer

Andrews, David

Two-photon fluorescence resonance energy transfer in protein-fluorophore complexes

Kam Z.

Crossref

A microscopic theory of two-photon fluorescence energy transfer is presented with particular view to its use as a probe into the structure of protein-fluorophore complexes. The onset of a radiative character to the energy transfer is identified at comparatively short distances, and is apparent in substantially modified fluorescence polarization.</jats:p

Philip Allcock

David L. Andrews

Two-Photon Fluorescence Resonance Energy Transfer in Protein-Fluorophore Complexes

https://ueaeprints.uea.ac.uk/56504/1/103.pdf

Two-photon fluorescence: Resonance energy transfer

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University of East Anglia digital repository

University of East Anglia digital repository

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