Chetverin, Alexander B.

Kramer, Fred R.

Kurnasov, Oleg V.

Morozov, Igor

Ryabova, Lyubov A.

Spirin, Alexander S.

Ugarov, Viktor I.

Volianik, Elena V.

Wu, Ying

Zhang, David

Batch and continuous-flow cell-free methods for synthesizing protein wherein translation is coupled with replication of recombinant mRNA by an RNA-directed RNA polyerase, such as Qβ replicase. Transcription of a DNA template to produce the recombinant mRNA can be coupled additionally with coupled transcription-replication-translation methods, both batch and continuous. The invention also includes kits of reagents for synthesizing user-selected proteins according to methods of this invention

Coventry University Pure Portal

Coupled replication-translation methods and kits for protein synthesis

Coupled replication-translation methods and kits for protein synthesis

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Coventry University Pure Portal