There is a weight issue weighing down the public health care systems across the world. Although obesity is prevalent in both males and females, the location of fat and impact on cardiometabolic health is strikingly different. These differences are apparent in the clinical setting, but there remains a bias towards using a single sex in mouse models to create simpler and cheaper experiments. The bias towards using a single sex in experiments skews the results and only offers translational research to one half of the human population. We examined sex differences and their effect on obesity by inducing obesity in both male and female mice by feeding them high fat diet (HFD) for 10 weeks. Mice were weighed weekly and after 10 weeks of HFD feeding, mice underwent metabolic tests to determine the impact of obesity. Male mice became obese after only 1 week of HFD feeding, however it took female mice 9 weeks of HFD feeding to become obese. Male mice were more susceptible to diabetes and male mice lost increased metabolic difference when fed HFD. This study highlights the importance of using both sexes to study obesity and associated diseases while highlighting novel differences in metabolism between sexes
