Background: High epidemiology of influenza in the world and Indonesia causes some groups to have high risk for getting infected with influenza, one of which is healthcare workers. The low rates of influenza vaccination among healthcare workers, especially in nurses, cause the need for an educational strategy to prevent influenza. This study aimed to measure the level of knowledge, attitude and practice among nurses in suburban hospital in West Java.Method: This was a cross-sectional study with random sampling method which started on July-August 2018 at Annisa Medical Center General Hospital. Knowledge, attitude, and practice of research subjects were measured using questionnaire containing 37 questions. The questionnaire sheet is the result of adaptation of several questionnaires and validity test has been conducted. The data obtained was then analyzed descriptively by the proportion of the score of knowledge, attitude, and practice.Result: Of 104 respondents, there were 19% respondents had low level, 74% respondents had moderate level and 7% respondents had high level of knowledge toward influenza vaccination. For attitude, 67% respondents had positive attitude and 33% respondents had negative attitude. The study also showed 58% respondents had positive practice and 42% respondents had negative attitude.Conclusion: Nurses possess adequate knowledge and they showed positive attitude and practice toward influenza vaccination. Continuous educational strategy was needed to improve nurses’ knowledge, attitude and practice on influenza vaccination. Keywords: Attitude, influenza vaccination, knowledge, nurses, practic