With propagating through a dispersive medium, the temporal-spectral profile
of laser pulses should be inevitably modified. Although such dispersion effect
has been well studied in classical optics, its effect on a single-photon
wave-packet, i.e., the matter wave of a single-photon, has not yet been
entirely revealed. In this paper, we investigate the effect of dispersion on
indistinguishability of single-photon wave-packets through the Hong-Ou-Mandel
(HOM) interference. By dispersively manipulating two indistinguishable
single-photon wave-packets before interfering with each other, we observe that
the difference of the second-order dispersion between two optical paths of the
HOM interferometer can be mapped to the interference curve, indicating that (1)
with the same amount of dispersion effect in both paths, the HOM interference
curve must be only determined by the intrinsic indistinguishability between the
wave-packets, i.e., dispersion cancellation due to the indistinguishability
between Feynman paths; (2) unbalanced dispersion effect in two paths cannot be
cancelled and will broaden the interference curve thus providing a way to
measure the second-order dispersion coefficient. Our results suggest a more
comprehensive understanding of the single-photon wave-packet and pave ways to
explore further applications of the HOM interference