Dwek, Raymond A.

Ferguson, Michael A. J.

Moxon, E. Richard

Rademacher, Thomas W.

Zamze, Susanne E.

A phosphorylated 3-deoxy-manno-octulosonic acid (KDO) was released from the lipopolysaccharide (LPS) of the deep rough mutant (Rb+169) of Haemophilus influenzae by acid hydrolysis. Both phosphorylated and dephosphorylated KDO, produced by treatment with alkaline phosphatase, were identified by gas chromatography-mass spectrometry after trimethylsilylation. This technique provides a rapid and reliable method for the identification of phosphorylated KDO in LPS.</p

Identification of phosphorylated 3-deoxy-<em>manno</em>-octulosonic acid as a component of <em>Haemophilus influenzae</em> lipopolysaccharide.

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University of Dundee Online Publications

University of Dundee Online Publications