An amendment to this paper has been published and can be accessed via a link at the top of the paper

Aflalo, Aure

Ahmed, Ibrahim

Bashford-Rogers, Rachael JM

Smith, Kenneth GC

Tucci, Felicia A

The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library

Tucci, Felicia Anna

Smith, Kenneth G. C.

Bashford-Rogers, Rachael

ORA - Oxford University Research Archive

Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction

Abstract: The ability to accurately characterize DNA variant proportions using PCR amplification is key to many genetic studies, including studying tumor heterogeneity, 16S microbiome, viral and immune receptor sequencing. We develop a novel generalizable ultrasensitive amplicon barcoding approach that significantly reduces the inflation/deflation of DNA variant proportions due to PCR amplification biases and sequencing errors. This method was applied to immune receptor sequencing, where it significantly improves the quality and estimation of diversity of the resulting library

Tucci, Felicia A.

Bashford-Rogers, Rachael J. M.

Apollo (Cambridge)

English

Ultrasensitive amplicon barcoding for next-generation sequencing facilitating sequence error and amplification-bias correction.

Oxford University Research Archive

1Vol.:(0123456789)Scientific RepoRtS |        (2020) 10:17010  | https://doi.org/10.1038/s41598-020-74321-4www.nature.com/scientificreportsAuthor correction: Ultrasensitive amplicon barcoding for next‑generation sequencing facilitating sequence error and amplification‑bias correctionibrahim Ahmed, felicia A. tucci, Aure Aflalo, Kenneth G. c. Smith & Rachael J. M. Bashford‑RogersCorrection to: Scientific Reports https ://doi.org/10.1038/s4159 8-020-67290 -1, published online 29 June 2020A supplementary file containing Supplementary Figures S1-S8 was omitted from this Article. This file is linked to this correction notice.Additional informationSupplementary information is available for this paper at https://doi.org/10.1038/s41598-020-74321-4.Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creat iveco mmons .org/licen ses/by/4.0/.© The Author(s) 2020open