Thyroid metastasis is rarely diagnosed, and the treatment outcomes in p16-positive oropharyngeal squamous cell carcinoma patients with rare thyroid metastasis have not been fully investigated. Here we describe the case of a patient with p16-positive oropharyngeal squamous cell carcinoma who was diagnosed with cT4 N2M1 with rare thyroid metastasis. The patient was a current smoker and was positive for human papillomavirus DNA, with disease progression at 49 days and death at 113 days after completion of cisplatin-based concurrent chemoradiotherapy

Beppu, Shintaro

Hanai, Nobuhiro

Koide, Yusuke

Nishikawa, Daisuke

Sasaki, Eiichi

Suzuki, Hidenori

Terada, Hoshino

English

Tokushima University Institutional Repository

CASE REPORTp16-positive oropharyngeal cancer with rare thyroid metastasis :A case reportHidenori Suzuki1, Eiichi Sasaki2, Daisuke Nishikawa3, Daisuke Nishikawa1, Yusuke Koide1, Shintaro Beppu1, Hoshino Terada1, and Nobuhiro Hanai11Department of Head and Neck Surgery, 2Department of Pathology and Molecular Diagnostics, Aichi Cancer Center Hospital, Nagoya, Japan, 3Department of Otolaryngology-Head and Neck Surgery, Nara Medical University, Kashihara, JapanAbstract : Thyroid metastasis is rarely diagnosed, and the treatment outcomes in p16-positive oropharyngeal squamous cell carcinoma patients with rare thyroid metastasis have not been fully investigated. Here we de-scribe the case of a patient with p16-positive oropharyngeal squamous cell carcinoma who was diagnosed with cT4 N2M1 with rare thyroid metastasis. The patient was a current smoker and was positive for human papil-lomavirus DNA, with disease progression at 49 days and death at 113 days after completion of cisplatin-based concurrent chemoradiotherapy. J. Med. Invest. 67 : 189-191, February, 2020Keywords : thyroid metastasis, oropharyngeal squamous cell carcinoma, p16-positive, HPV-DNA, smoking statusINTRODUCTION p16 was established as a surrogate immunohistochemical marker of human papillomavirus (HPV) –mediated oropharyn-geal squamous cell carcinoma (OPSCC) in the eighth edition of the American Joint Committee on Cancer Staging Manual (1). Previous studies reported that patients with p16-positive OPSCC had better survival outcomes than those with p16-nega-tive OPSCC (2-6). Metastasis of the thyroid gland is rarely diag-nosed in the clinical practice ; only 46 patients with rare thyroid metastasis were found in 18,105 thyroidectomies and 29,708 cy-tological examination over a 10-years period (9). Moreover, there were no mention of thyroid metastasis in a systematic review of the end organs of distant metastasis in 220 patients developing of HPV-positive OPSCC (3). Therefore, the treatment outcomes of p16-positve OPSCC with rare thyroid metastasis remain un-clear (7). Here we report the survival outcomes of a patient with p16-positve OPSCC with rare thyroid metastasis. CASE PRESENTATIONA 69-year-old man with a 3-year history of hoarseness and dysphagia was referred to our hospital after the previous hos-pital diagnosed him with pharyngeal squamous cell carcinoma based on biopsy. The patient was a 49 pack-year current smoker according to a previous report (2). The habit of alcohol consump-tion was once a week for 40 years. Bilateral neck lymph nodes were palpable. Inspection and endoscopy revealed that the pha-ryngeal tumor had spread to the left tonsil, base of the tongue, left pyriform sinus, and lingual surface of the epiglottis ; bilater-al recurrent nerves palsy was also noted. Enhanced/plane com-puted tomography (CT) performed from the cervix to the pelvis revealed an oropharyngeal tumor at the base of the tongue. The tumor had spread to the extrinsic muscle of the tongue, and the main lesion had a maximum size of 60 mm, with bilateral neck metastasis not larger than 30 mm and heterogeneity of bilateral thyroid (Figure 1). The patient was hospitalized for narrow air-way and dysphagia upon his first visit to our hospital. After hospitalization, p16 immunohistochemistry (CINtec Histology Kit, clone E6H4, Ventana, Tucson, AZ, USA) of the oropharyngeal tumor was performed using an automatic ma-chine (Ventana BenchMark XT), which indicated that the tumor had diffused and was strongly positive. Echo-guided cytology of the bilateral thyroid indicated metastatic squamous cell carcino-ma (Figure 2). Thus, p16-positive OPSCC of cT4N2M1 with rare thyroid metastasis was diagnosed based on the 8th edition of the American Joint Committee on Cancer Staging Manual (1). Cisplatin-based concurrent chemoradiotherapy (CRT) for organ preservation definitively underwent after gastrostomy The Journal of Medical Investigation    Vol. 67  2020　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　Received for publication May 20, 2019 ; accepted August 9, 2019.Address correspondence and reprint requests to Hidenori Suzuki, Department of Head and Neck Surgery, Aichi Cancer Center Hos-pital, 1-1 Kanokoden, Chikusa-ku, Nagoya 464-8681, Japan and Fax : +81-52-764-2963.Figure 1.　(A) White light and (B) narrow-band endoscopy images as well as (C) axial oropharyngeal, (D) axial thyroid, and (E) coronal thy-roid enhanced computed tomography images in oropharyngeal cancer.189190 H. Suzuki, et al.  Thyroid metastasis of p16-positive OPSCCand tracheostomy for hypoxemia. A total of 70 Gy with 2 Gy/daily fraction was performed by intensity-modulated radio-therapy (RT) following three-dimensional conformal RT to the whole neck. Cisplatin (one cycle of 100mg/m2) was administered triweekly to the patient ; however, the dosage was reduced to two to three cycles of 80 mg/m2 because of febrile neutropenia. The first hospitalization period lasted 75 days. At 49 days following the completion of CRT, enhanced CT re-vealed tumor reduction in the RT field as well as multiple distant lymph node metastases affecting the mediastinum, hilum, and axilla in areas other than the RT field. Additionally, echo-guided cytology of the axillary lymph node revealed metastatic squa-mous cell carcinoma (Figure 3). The patient was diagnosed with disease progression due to multiple distant metastases, and the best supportive care was selected owing to general fatigue. The patient was subsequently re-hospitalized because of dyspnea due to pleural effusion and carcinomatous carcinomatosa, and eventually died. The re-hospitalization period lasted 7 days. The durations from pathological diagnosis and completion of CRT to death were 197 days and 113 days, respectively. HPV DNA-pos-itive was detected in formalin-fixed, paraffin-embedded tumor specimen by HPV testing using GP5+/GP6+ PCR, according to a previous report (8). HPV 16 was identified based on the L1 sequence (Figure 4).DISCUSSIONRare thyroid metastasis, accounting for 0.13 % of all thyroidec-tomies (9), has been reported as a case report in various tumors such as neuroendocrine tumor from our hospital (10). Thyroid metastasis of p16-positve OPSCC is rare and has unknown survival outcomes (7). A recent systemic review of 453 articles, including 220 cases developing distant metastasis of OPSCC with HPV, revealed that the end organs of distant metastasis were the lung (160/220), bone (39/220), and non-regional lymph nodes (34/220) ; however, no rare thyroid metastasis was report-ed (3). Moreover, the survival outcomes of two cases with thyroid metastasis among 80 cases with recurrent p16-positive OPSCC were unclear (4). Among patients without distant metastasis, those with p16-positive OPSCC had better outcomes than those with p16-negative OPSCC (2-4, 6). Moreover, among patients with distant metastasis, those with p16-positive OPSCC (28% at 1-year) had significantly better post-distant metastasis survival rate than those with p16-negative OPSCC (13% at 1-year) (5). As a clinical predictor in patients with p16-positive OPSCC, those with current smoker showed worse outcomes than those with never smoking history (2, 6). A large cohort study demon-strated in 2018 that 48 patients with p16-positive OPSCC who were negative for HPV DNA had significantly shorter survival outcomes than 340 patients who were positive for HPV DNA and had similar survival outcomes as 816 patients with p16-negative OPSCC (12). Considering the clinical stage and treatment strategy in a recent and similar case with a favorable course of p16-positive OPSCC with rare thyroid metastasis that did not assess HPV DNA and smoking status, no tumor recurrence observed 6 months after total thyroidectomy following definitive CRT (7). In contrast to the previous report (7), our patient presented an unfavorable course in terms of disease progression at 49 days and death at 113 days following definitive CRT. Because p16 positivity was a surrogate marker of HPV (11), the present case was examined for p16-positive OPSCC with HPV-negative DNA. Although the HPV DNA status was not reported in the previous case (7), the present case was both p16 and HPV DNA positive. Our study suggests the patient’s current smoking status possibly led to the unfavorable outcome. We believe that this patient’s poor prognosis was strongly affected by his current smoking status, which has been independently indicated as a poor predic-tor for OPSCC in a randomized trial (6). Further accumulation Figure 2.　(A) Hematoxylin and eosin staining as well as (B) p16 immunohistochemical sections for oropharyngeal tumor and (C) right and (D) left Papanicolaou staining sections of the thyroid.Figure 3.　(A) Axial enhanced computed tomography image and (B) Papanicolaou staining of the axillary lymph node.Figure 4.　Polymerase chain reaction gel migration of GP5+/GP6+ primers from the oropharyngeal tumor.191The Journal of Medical Investigation   Vol. 67  February  2020of cases of p16-positive OPSCC with rare thyroid metastasis, including smoking status and HPV DNA, is required. Although alcohol consumption has been reported to negatively influence survival (1), we were unable to suggest the role of alcohol con-sumption in the development and progression of the cancer in this patient.CONCLUSIONWe report that case of a patient with p16-positive OPSCC with rare thyroid metastasis and current smoking status who was positive for HPV DNA. The patient exhibited disease progres-sion at 49 days and died 113 days after CRT.CONFLICT OF INTERESTAll authors declare that they have no conflict of interest.ACKNOWLEDGMENTSThe authors would like to thank Dr. Yutaro Koide for stimulat-ing discussion and patient’s care. REFERENCES1. Amin MB, Edge SB, Greene FL, Byrd DR, Brookland RK, Washington MK, Gershenwald JE, Compton CC, Hess KR, Sullivan DC, Jessup JM, Brierley JD, Gaspar LE, Schilsky RL, Balch CM, Winchester DP, Asare EA, Madera M, Gress DM, Meyer LR : AJCC Cancer Staging Mannual, 2016 8th edition. Springer ; New York.2. Vawda N, Banerjee RN, Debenham BJ : Impact of smoking outcomes of HPV-related oropharyngeal cancer treated with primary radiation or surgery. Int J Radiat Oncol Biol Phys 103(5) : 1125-1131, 20193. 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Thyroid metastasis of p16-positive OPSCC

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Thyroid metastasis of p16-positive OPSCC

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