Alberto, Lapini

Alexander, Kutikov

Campi, Riccardo

Carini, Marco

Ilaria, Montagnani

Mari, Andrea

Maria Rosaria Raspollini,

Minervini, Andrea

Smaldone, Marc C.

Uzzo, Robert G.

English

Florence Research

Patients were classified in relation to BMI. Blood samples forthe evaluation of adiponectin, leptin and MMP-3 werecollected. A 12-core transrectal prostate biopsy was performed.Serum adiponectin, leptin and MMP-3 were measured using“Human Leptin Instant ELISA”, “Human Adiponectin ELISA”,“Human MMP-3 ELISA” kits, respectively. Statistical analysiswas performed to relate the plasmatic levels of the above-mentioned biomarkers to the presence of Gleason patterns 4and 5 at biopsy. Results: Fifty-six patients were enrolled.Median serum levels of leptin, adiponectin and MMP-3 were0.829 ng/ml, 1.72 ng/ml and 1.767 ng/ml, respectively. Inrelation to BMI class, the plasmatic levels of leptin and MMP-3 were higher in obese (p=0.02) and in normal-weight patients(p=0.02), respectively. No statistically significant differencewas detected in serum levels of leptin (p=0.18), adiponectin(p=0.68) and MMP-3 (p=0.49) between the 24 patients(42.8%) with diagnosis of PCa and the 30 patients (53.7%)with a negative biopsy. Comparing the levels of biomarkers in11/24 patients (45.8%) with Gleason 6 (3+3) and in 13/24(54.2%) showing Gleason patterns 4 and 5 at biopsy, again, nostatistically significant difference in leptin (p=0.4), adiponectin(p=0.6) and MMP-3 (p=0.5) levels was found. Conclusion: Inour preliminary study, we found increased plasmatic levels ofleptin and MMP-3 in obese and normal-weight patientsundergoing prostate biopsy, respectively. The significance ofthis finding, in patients with an elevated PSA, is uncertain. Onthe other hand, no other statistical difference was foundbetween BMI, plasmatic levels of leptin, adiponectin, MMP-3and detection of an aggressive Gleason pattern at biopsy.We wish to thank the GSTU Foundation for the administrativesupport.81BEYOND THE COMPLEXITY OF TUMOR EXCISION DURING PARTIAL NEPHRECTOMY:IDEATION AND HISTOPATHOLOGICALVALIDATION OF THE SURFACE-INTERMEDIATE-BASE (SIB) MARGIN SCOREAbstracts of the 26th Annual Meeting of the Italian Society of Uro-Oncology (SIUrO), 9-11 June, 2016, Florence, Italy2599Figure 1. 360 overall histological measures.Andrea Minervini1, Riccardo Campi1, Maria RosariaRaspollini2, Andrea Mari1, Ilaria Montagnani2, Marc C. Smaldone3, Robert G. Uzzo3, Alberto Lapini1,Marco Carini1 and Alexander Kutikov3Departments of 1Urology and 2Pathology, University of Florence, Careggi Hospital, Florence, Italy, Firenze (FI), Italy;3Division of Urologic Oncology, Fox Chase Cancer Center, Philadelphia, PA, U.S.A.Introduction/Aim: Tumor excision is a fundamental step duringpartial nephrectomy (PN), yet resection technique (RT) israrely reported in current nephron-sparing surgery (NSS)literature. We recently proposed the Surface-Intermediate-Base(SIB) margin score as a new classification model forstandardized reporting of RT during NSS. The aim of the studywas to validate the SIB model from a histopathologicalperspective. Materials and Methods: Data were prospectivelycollected from a cohort of 40 patients undergoing NSS,between June and September 2014, at a single Institution. TheSIB score was assigned in the operating room by the surgeon.The score-specific areas (SSA) were outlined on a digitalpicture as anatomic landmarks for histopathological analysis.Two dedicated uropathologists inked the landmark areas andmeasured, in a blinded fashion, the maximum, minimum andmean thickness of healthy renal margin (HRM) within theSSAs (360 overall histologic measures, Figure 1). The Mann-Whitney U-test was used to assess the correlation between theSIB visual definitions of RTs and the thickness of HRM athistopathological analysis. Results: The overall RT wasclassified as pure enucleation, hybrid enucleation and pureenucleoresection in 28 (70%), 7 (17%) and 3 (7%) patients,respectively, while as hybrid enucleoresection and resection in1 (3%) patient each. At histopathological analysis, themaximum, minimum and mean thickness of HRM wassignificantly different among SSAs visually defined asenucleation (S=0: median=0.18mm (interquartile range(IQR)=0.08-0.30), I or B=0: median=0.20 mm (IQR=0.08-0.36)), enucleoresection (S=1: median=0.80 mm (IQR=0.67-1.16), I, B=1: median=0.88 mm (IQR=0.60-1.00)) andresection (S=1, I, B=2: median=2.95 mm (IQR=2.18-5.75)ANTICANCER RESEARCH 36: 2535-2628 (2016)2600Figure 2. Box-plots showing the minimum (Min), mean and maximum (Max) thickness of healthy renal margin (HRM) for the score-specificarea (SSA) – grades Surface=0 (S=0), Surface=1 (S=1), Intermediate or Base=0 (I or B=0), Intermediate or Base=1 (I or B=1) andIntermediate or Base=2 (I or B=2). Median values and interquartile ranges (white boxes) are shown. Thickness of HRM was significantlydifferent between both the SSA-grades S=0 and S= 1 (p<0,001) and the SSA-grades I or B=0, I or B=1 and I or B=2 (p<0,001), for allhistological measures (maximum, minimum and mean values). S, surface; I, intermediate; B, base.(Figure 2) (p<0.001). Conclusion: The SIB margin score is thefirst standardized reporting system to communicate RT duringNSS. Our study proved the applicability of the model in a real-world clinical setting and provided robust histopathologicalvalidation of its utility. 82A WHOLE TOMATO-BASED DIETARYSUPPLEMENT TO COMPLEMENT THE OUTCOMESOF THE WCRF/AICR RECOMMENDATIONSMauro Piantelli1, Vincenzo Fogliano2, Luigi Di Cormio3,Oscar Selvaggio3, Giuseppe Carrieri3, Stefano Iacobelli4and Pier Giorgio Natali51Department of Medical, Oral and OdontostomathologicSciences, University "G. D’Annunzio” of Chieti-Pescara, Chieti (CH), Italy;2Food Quality and Design, Wageningen University, Wageningen, Netherlands;3Urologic Clinic, University of Foggia Foggia, Italy;4Medical Oncology, University “G. D’Annunzio” of Chieti-Pescara, Chieti, Italy;5Immunology, MediaPharma Srl, Chieti, ItalyIntroduction: Despite differences in outcomes, due toheterogeneity in study designs, a wealth of clinical andexperimental evidences underscore the beneficial effects ofthe consumption of lycopene-rich tomatoes on prostatefunctions (1). Such effect, which is maximally reached usingcooked tomatos, has been shown to be dose-dependent (2).Thus, development of tomato-processing methods aimed atoptimally preserving the health-promoting activity of this fruitthat is of major translational relevance. Materials andMethods: Using the transgenic adenocarcinoma of the mouseprostate (TRAMP) model, the effect of a diet enriched withprocessed whole tomato on animal survival, tumorigenesisand progression was investigated. Results: Tomato-enricheddiet significantly increased overall survival, delayedprogression from prostatic intraepithelial neoplasia toadenocarcinoma and decreased the incidence of poorlydifferentiated carcinoma. This was paralleled by an increaseof plasma antioxidant capacity and a reduction of circulatingpro-inflammatory and pro-angiogenic cytokines of knownrelevance in human prostate carcinogenesis. Based on thesepreclinical data, we have developed a dietary supplementcontaining a blend of ad-hoc processed whole tomato andolive vegetation water for human use, called Lycoprozen®(Italian Health Ministry, code 68843) (3). Conclusion: Thisnew dietary supplement may help to maintain prostate healthand can contribute to the beneficial effect of adherence to theWCRF/AICR recommendations, especially when proper lifestyles are adopted.1 Raiola A, Rigano MM, Calafiore R, Frusciante L andBarone A: Enhancing the Health-Promoting Effects ofTomato Fruit for Biofortified Food. Mediators Inflamm2014: 139873, 2014. 2 Er V, Lane JA, Martin RM, Emmett P, Gilbert R, Avery KN,Walsh E, Donovan JL, Neal DE, Hamdy FC and Jeffreys M:Adherence to dietary and lifestyle recommendations andprostate cancer risk in the prostate testing for cancer andtreatment (ProtecT) trial. Cancer Epidemiol Biomarkers Prev23: 2066-2077, 2014.3 Tomato powder based composition. PCT, Publication No.WO/2015/044134 A1.83LIMITS OF TRANSURETHRAL RESECTION INDETECTING RARE HISTOLOGICAL VARIANTSWITHIN LARGE UROTHELIAL BLADDER TUMORSAlessio Guarneri1, Cristina Scalici Gesolfo1, Fabrizio Di Maida1, Giovanni Caruana1, Sebastiano Billone1, Marco Moschini2, Renzo Colombo2, Alchiede Simonato1and Vincenzo Serretta11Department of Surgical, Oncological and StomatologicalSciences, Section of Urology, University of Palermo,Palermo (PA), Italy;2Department of Urology, University "Vita Salute" SanRaffaele, Milano, ItalyIntroduction/Aim: Rare histotypes represent almost 10% ofbladder tumors, although more often represented as small fociwithin large and invasive transitional cell tumors of the bladder(TCCB). It might be clinically relevant that rare histologicalvariants remain unrecognized at transurethral resection(TURBT), since they could indicate more aggressive tumors,less responsive to systemic chemotherapy and unfit for “organ-sparing” management. We investigated the accuracy of TURBTto detect rare histological variants in patients-candidates tocystectomy for bladder cancer with clinical and radiologicalfeatures of invasiveness. Materials and Methods: The clinicaland pathologic data of 340 patients submitted to TURBT and/orcystectomy for bladder cancer, between January 2010 and July2015, were reviewed. The presence of uncommon histotypeswithin urothelial bladder carcinoma has been assessed. Thediagnosis of rare variants of bladder cancer was made accordingto WHO criteria. Standard hematoxilyn-eosin stain was adoptedand further immunohistochemistry was performed as follows:Micro-papillary carcinoma, MUC1, EMA, CK7, CK20;Squamous cell carcinoma, CK5/6, CK5/14; Adenocarcinoma,CK7, CK20, CEA, EMA; Small cell carcinoma, EMA,CAM5.2, synaptophysin, vimentin, chromogranin, neuro-specific enolase (NSE), CK7, c-kit and TTF1; MesenchymalAbstracts of the 26th Annual Meeting of the Italian Society of Uro-Oncology (SIUrO), 9-11 June, 2016, Florence, Italy2601

BEYOND THE COMPLEXITY OF TUMOR EXCISION DURING PARTIAL NEPHRECTOMY:IDEATION AND HISTOPATHOLOGICAL
VALIDATION OF THE SURFACE-INTERMEDIATEBASE (SIB) MARGIN SCORE

https://flore.unifi.it/bitstream/2158/1056672/1/81.pdf

BEYOND THE COMPLEXITY OF TUMOR EXCISION DURING PARTIAL NEPHRECTOMY:IDEATION AND HISTOPATHOLOGICAL

VALIDATION OF THE SURFACE-INTERMEDIATEBASE (SIB) MARGIN SCORE

BEYOND THE COMPLEXITY OF TUMOR EXCISION DURING PARTIAL NEPHRECTOMY:IDEATION AND HISTOPATHOLOGICAL VALIDATION OF THE SURFACE-INTERMEDIATEBASE (SIB) MARGIN SCORE

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