Ivanova, P.

Mitev, V.

Poumay, Y.

English

Immortalized hTert keratinocytes have been obtained from normal human epidermal keratinocytes, which were infected with amphotropic retroviral vectors encoding hTert, the catalytic subunit of telomerase, followed by spontaneous loss of p16INK4a function. There are no any data in the literature concerning transduction pathways participating in regulation of proliferation and/or differentiation of this cell line, probably because it was previously supposed that immortalization of the cells does not disrupt other normal growth control mechanisms. In this study, we explored the effects of PKD1 inhibition in hTert keratinocytes, using siRNA approach, on expression and activation of ERK1/2 and EGFR which acts to promote proliferation and survival in normal human epidermal keratinocytes. Treatment of cultured hTert keratinocytes with siRNA for PKD1 effectively diminished protein levels of the kinase, ERK1/2 expression and activity, EGFR expression and activity and according to our previous results in K10 and involucrin expression. It is possible that PKD1, ERK1/2 and EGFR have adverse effects in immortalized human hTert keratinocytes compared to normal human epidermal keratinocytes in regulation of proliferation and differentiation. We showed for the first time that ERK1/2 MAPK, which are considered to be constitutively expressed kinases in mammalian cells, are inducible enzymes regulated by PKD1 in hTert keratinocytes.</p

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RESEARCH OUTPUTS / RÉSULTATS DE RECHERCHEAuthor(s) - Auteur(s) :Publication date - Date de publication :Permanent link - Permalien :Rights / License - Licence de droit d’auteur :Bibliothèque Universitaire Moretus PlantinInstitutional Repository - Research PortalDépôt Institutionnel - Portail de la Rechercheresearchportal.unamur.beProtein kinase D1 upregulates expression and activity of extracellular signal-regulatedkinase 1/2 and EGFR in human hTert keratinocytesIvanova, P.; Poumay, Y.; Mitev, V.Published in:Comptes Rendus de L'Academie Bulgare des SciencesPublication date:2007Document VersionPublisher's PDF, also known as Version of recordLink to publicationCitation for pulished version (HARVARD):Ivanova, P, Poumay, Y & Mitev, V 2007, 'Protein kinase D1 upregulates expression and activity of extracellularsignal-regulated kinase 1/2 and EGFR in human hTert keratinocytes', Comptes Rendus de L'Academie Bulgaredes Sciences, vol. 60, no. 7, pp. 783-788.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            • Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            • You may not further distribute the material or use it for any profit-making activity or commercial gain            • You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Download date: 23. Jun. 2020

Protein kinase D1 upregulates expression and activity of extracellular signal-regulated kinase 1/2 and EGFR in human hTert keratinocytes

https://pure.unamur.be/ws/files/12379804/Ivanova2007_CRAcadSciBulg60_783.pdf

Protein kinase D1 upregulates expression and activity of extracellular signal-regulated kinase 1/2 and EGFR in human hTert keratinocytes

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