Atanasova, G.

Isaeva, A.

Ivanova, P.

Kalenderova, S.

Mitev, V.

Poumay, Y.

English

In the present study we have analysed the effect of the pharmacological CDK-inhibitor CYC202 on HPV16 E6- and E7-transformed human keratinocytes. The proliferation rates of E6E7 keratinocytes treated with CYC202 were determined by measurement the 3H-thymidine incorporation in the newly synthesised DNA molecules. The effect of CYC202 on some mitogen-activated protein kinases (MAPK) implicated in the regulation of cell proliferation, differentiation and apoptosis was also studied by Western blotting. CYC202 effectively inhibits the proliferation of E6E7 keratinocytes in a dose-dependent manner. Treatment with CYC202 strongly increases the activity of p38 MAP kinase and inhibits ERK1/2 at the highest concentration used. The phosphorylation of HSP27 and c-Myc are also changed in correlation with the upstream kinases from the MAPK family.</p

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RESEARCH OUTPUTS / RÉSULTATS DE RECHERCHEAuthor(s) - Auteur(s) :Publication date - Date de publication :Permanent link - Permalien :Rights / License - Licence de droit d’auteur :Bibliothèque Universitaire Moretus PlantinInstitutional Repository - Research PortalDépôt Institutionnel - Portail de la Rechercheresearchportal.unamur.bePharmacological growth inhibition of papillomavirus type 16 E6E7-transformedkeratinocytes by the CDK-Inhibitor CYC202Atanasova, G.; Isaeva, A.; Ivanova, P.; Kalenderova, S.; Poumay, Y.; Mitev, V.Published in:Comptes Rendus de L'Academie Bulgare des SciencesPublication date:2007Document VersionPublisher's PDF, also known as Version of recordLink to publicationCitation for pulished version (HARVARD):Atanasova, G, Isaeva, A, Ivanova, P, Kalenderova, S, Poumay, Y & Mitev, V 2007, 'Pharmacological growthinhibition of papillomavirus type 16 E6E7-transformed keratinocytes by the CDK-Inhibitor CYC202', ComptesRendus de L'Academie Bulgare des Sciences, vol. 60, no. 2, pp. 183-188.General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.            • Users may download and print one copy of any publication from the public portal for the purpose of private study or research.            • You may not further distribute the material or use it for any profit-making activity or commercial gain            • You may freely distribute the URL identifying the publication in the public portal ?Take down policyIf you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediatelyand investigate your claim.Download date: 23. Jun. 2020

Pharmacological growth inhibition of papillomavirus type 16 E6E7-transformed keratinocytes by the CDK-Inhibitor CYC202

https://pure.unamur.be/ws/files/12379752/Atanasova2007_CRAcadSciBulg60_183.pdf

Pharmacological growth inhibition of papillomavirus type 16 E6E7-transformed keratinocytes by the CDK-Inhibitor CYC202

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