340–343Staphylococcus aureus (S. aureus) is the most common reason behind bacterial infections in humans. They cause a wide array of infections on skin, bone and joint medical implants and blood stream. In order to mitigate these infections, detecting the presence of this bacterium is of prime importance. This paper explores a new rapid detection technique to identify S aureus bacteria. A screen-printed sensor coated with graphene oxide was used as an identification platform. Graphene oxide was synthesized using modified Hummer’s method and was characterized using X-ray Diffractometer and Scanning Electron Microscope. The developed sensors showed significant decrease in the bulk resistance with the increase in bacterial concentrations. The developed sensors were subjected to selectivity, repeatability and reusability tests and showed good results