Stability Indicating RP-HPLC for Quantification Mangiferin in Extract of Three Species Mango Leaves

Abstract

The stability indication of Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) method was validated for quantitative determination of mangiferin on three species mango leaves (Mangifera odorata Griff, Mangifera foetida Lour, and Mangifera indica L.). The samples were extracted by maseration method using methanol and concentrated using rotary evaporator. The method carried out on stationary phase a purospher RP-18 endcapped (25 cm × 4.6 mm i.d., 5 µm) column with a mobile phase consisting of methanol: phosphoric acid 0.1% (v/v) (31:69); flow rate:0.8 mL/min; solvent methanol, detection was carried out at 258 nm. The analytical  performace this measurement is good with the value of linearity (r2=0.998), precision (%RSD=0.649%), and accuration (10.67%). The forced degradation studies were carried out according to the International Conference on Harmonization (ICH) guidelines. The results indicating that the complete separation between degradation products and mangiferin peak occured. The degradation limit of mangiferin 5–20% (according to the guideline of ICH) except in basic condition (100%). The method was succesful applied to determine of the mangiferin in  pakel (Mangifera foetida), kweni (Mangifera indica) and kopyor (Mangifera odorata) extract. The mangiferin content was obtained are pakel (9.95%), kopyor (7.40%) and kweni (Mangifera odorata) (2.49%) respectively.Makalah ini menyajikan analisis mangiferin pada tiga spesies daun mangga (Mangifera odorata Griff .; Mangifera foetida Lour .; Mangifera indica L.) menggunakan Kromatografi Cair Kinerja Tinggi Fase Balik (RP-HPLC). Setiap sampel diekstraksi secara maserasi dengan pelarut metanol  dan dipekatkan menggunakan rotary evaporator. Metode analisis, menggunakan sistem RP-HPLC dengan kondisi analisis sebagai berikut fase gerak, metanol: asam fosfat 0,1% (v / v) (31:69); fase diam RPC-18; laju alir: 0,8 ml / menit; pelarut metanol; Panjang gelombang 258 nm. Hasil validasi menunjukkan linearitas yang baik  (r = 0,998), presisi (% RSD = 0,649%), dan akurasi (101,67%). Setelah proses validasi, ekstrak daun mangga pakel (Mangifera foetida) dan kopyor (Mangifera odorata) dianalisis dan spesies yang mengandung  mangiferin dengan kadar tertinggi  adalah pakel (9,95%) diikuti oleh kopyor (7,40%) dan kweni (Mangifera odorata) (2,49%). Studi degradasi paksa juga dilakukan sesuai pedoman ICH. Hasil Uji degradasi paksa menunjukkan adanya pemisahan yang sempurna atara hasil degradasi dan puncak mangiferin.Degradasi mangiferin berada dalam batas (5-20%, sesuai dengan pedoman ICH) kecuali dalam kondisi basa, degradasinya mencapai 100%. &nbsp