Class switching of an anti-FluB broad-spectrum IgM mAb via activation-induced cytidine deaminase (AID) activity and the preliminary functional study of these IgG mAbs
流感病毒是威胁人类健康和公共卫生的一大重要问题，其中乙型流感(FluB)就是一种引发流感流行的主要病原体。由于流感病毒的持续快速变异，针对其的治疗药物必须具备广谱性。FluB的广谱中和单克隆抗体，尤其IgG型抗体是防治乙型流感病毒的一种潜在的有效手段。 本实验室在前期获得一株具有FluB广谱中和活性的IgM型鼠源单克隆抗体7G1。与传统IgG治疗性抗体药物相比，IgM抗体存在成产及纯化工艺较为复杂、抗体介导的杀伤作用较弱、半衰期较短等缺点。本研究开展了一种不依赖于抗体可变区基因克隆的IgG类别转换方法。我们通过在杂交瘤细胞中构建可调控表达活化诱导胞嘧啶核苷脱氨酶(activation-ind...Influenza virus is one of the most important public health issues with high impact on human health, and Influenza B (FluB) is one of the main pathogens causing epidemics. While the rapid evolution of influenza virus making the development of broadly applicable antiviral drugs challenging. Broadly neutralizing monoclonal antibodies against Flu B, especially IgG antibodies, may show great potential ...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162014115246
