The Construction of On-line Sephadex LH-20 Liquid Chromatography × Reversed Phase Liquid Chromatography System and the Study on the Chemical Constituents of Flos Carthami
本研究针对二维液相在复杂样品分离中的不足，结合新的混合模式色谱，构建基于SephadexLH-20×RPLC联用的新型二维色谱方法体系，并探索该系统在中药材分析和分离制备上的应用。 构建SephadexLH-20与反相色谱联用的在线分析系统，以含多个化合物的模式混合物作为研究对象，通过探究各化合物的液相保留行为证明了葡聚糖凝胶在甲醇/水洗脱模式下可兼具尺寸排阻、氢键吸附、分配三种分离机制。以红花作为研究对象，在确定溶剂洗脱模式的基础上考察第一维SephadexLH-20柱的柱长、流速等因素对分离效果的影响，确定分离参数；选择适合该二维系统的接口；将构建的SephadexLH-20×RPLC联...A novel on-line comprehensive two-dimensional mixed-mode Sephadex LH-20 chromatography × reversed phase liquid chromatography (Sephadex LH-20 × RPLC) was constructed with the development of mixed-mode chromatography technique. Firstly, the mixture of reference compounds was separated on Sephadex LH-20 with methanol or methanol/water as elution solvent. Based on the retention time of these compou...学位：医学硕士院系专业：药学院_药物分析学学号：3232014115342
